黑米花青素在大鼠视网膜光化学损伤中的抗氧化作用研究  

THE ANTIOXIDATIVE EFFECTS OF ANTHOCYANIDINS FROM BLACK RICE ON RETINA PHOTOCHEMICAL DAMAGE IN RATS

作　　者：陈玮[1] 凌文华[2] 李茂全[1] 魏敏[1] 潘渠[1] 余小平[1] 

机构地区：[1]成都医学院公共卫生系,第一附属医院烧伤外科,成都610083 [2]中山大学公共卫生学院营养系,广州510080

出　　处：《营养学报》2010年第4期341-344,349,共5页Acta Nutrimenta Sinica

基　　金：国家自然科学基金(No.30972462);教育部新世纪优秀人才支持计划(No:NECT-08-0901)

摘　　要：目的探讨黑米花青素(anthocyanins-rich extract from black rice,BRACs)防护视网膜光化学损伤(retinal photochemical damage,RPD)的机制。方法 60只Sprague-Dawley大鼠饲以AIN-93基础饲料1w后,随机分为对照组(饲以基础饲料,n=10),光照组(饲以基础饲料,同时3 000±200 lux强度的白色荧光持续光照24h,n=25)和BRACs组(饲以基础饲料的同时,灌胃给予100mg BRACs/kg bw 15d,光照24h,n=25)。然后测定光照0,3,6,12,24h视网膜组织中脂质过氧化终产物丙二醛(malondialdehyde,MDA)含量和抗氧化酶系超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)和谷胱甘肽S-转移酶(glutathione S-transferase,GST)的活性。结果各组大鼠进食量和体重无显著统计学差异(P>0.05),光照后大鼠视网膜内MDA含量随光照时间逐渐增加,BRACs干预能降低光照大鼠MDA含量(P<0.05);同时光照降低了SOD、GSH-Px、GST活性,BRACs能升高光照大鼠视网膜抗氧化酶SOD和GSH-Px活性(P<0.05)。结论 BRACs防护视网膜光化学损伤与其较强的抗氧化特性有关。Objective To investigate the underlying mechanism involved in anthocyanins rich extract from black rice（BRACs） to protect retina from photochemical damage（RPD） in vivo.Method Sixty Sprague-Dawley rats fed with AIN-93 diet for 1w were randomly divided into normal control group（fed with AIN-93 diet,n=10）,1ight group（fed with AIN-93 diet and exposed to 3000 ± 200 lux fluorescent light for 24h,n=25）,and BRACs group（intragastric administration with 100mg BRACs/kg bw for 15d and exposed to light for 24h,n=25）.Thereafter,the concentration of malondialdehyde（MDA）,the activity of superoxide dismutase（SOD）,glutathione peroxidase（GSH-Px） and glutathione S-transferase（GST） were measured in retina at 0,3,6,12,24 h exposure time.Results No statistical difference in food-intake and body weight was observed among the three groups during the experiment period.The light exposure gradually caused MDA significantly increased in retina,but BRACs administration decreased the MDA level.Additionally,light exposure caused the activity of SOD,GSH-Px and GST significantly decreased in retina,and BRACs increased activity of antioxidases SOD and GSH-Px in retina.Conclusion The protective effect of BRAC from photochemical damage to retina is related to its potent antioxidative capability.

关 键 词：黑米花青素 大鼠 视网膜光损伤 抗氧化 

分 类 号：R285.5[医药卫生—中药学]