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作 者:李欣[1] 沈向群[1] 孙功[1] 王妍[1] 孙欣欣 马岳
机构地区:[1]沈阳农业大学园艺学院,沈阳110161 [2]沈北新区新城子现代副城开发区管委会,沈阳110161 [3]黑龙江新天物业集团,黑龙江齐齐哈尔161000
出 处:《西北农业学报》2010年第8期188-193,共6页Acta Agriculturae Boreali-occidentalis Sinica
基 金:沈阳市科技攻关项目(1053100-3-02)
摘 要:以两对隐性等位基因控制的闭花瓣型矮牵牛为母本,正常开放纯合型的矮牵牛为轮回父本,采用杂交、回交、测交验证和自交方法,保留闭花瓣型基因的同时获得不同颜色的闭花瓣型矮牵牛,并对不同回交世代数的植株进行植物学性状调查,确定最佳的回交代数。试验结果表明,在每一代回交后代中,注重同型株的选择,颜色、大小越接近轮回亲本,回交的效果越好,回交3代可以基本满足目标经济性状的要求。The female parent is a petunia with petals-closed flower controlled by two pairs of recessive alleles,and the recurrent male is a homozygous petunia with the normal opening flower.During the process of hybridization,backcross,testcross verification and selfing,the petals-closed flower trait was selected and petunia lines with petals-closed and color-differentiated flower were obtained.Botanical traits investigation was conducted in different backcross generations to determine the optimal generation of backcross.Experimental results showed that focusing on the selection of isoform plants,the closer in color and size of flower to the recurrent parent in each generation of backcross progeny,the better effect of backcross.Three generations of backcross can basically meet the requirements of economic traits.
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