补肾健脾对原因不明复发性流产患者血CD4^+ CD25^+调节性T细胞表达频率的研究  

Changes in expressions of CD4^+ and CD25^+ T regulatory cells after nourishing kidney and strengthing spleen for unexplained recurrent spontaneous abortion

作　　者：焦榕芳[1] 李爱青[1] 熊任英[1] 刘美珍[1] 

机构地区：[1]南昌大学第四附属医院妇产科,330003

出　　处：《实用医学杂志》2010年第18期3423-3425,共3页The Journal of Practical Medicine

基　　金：江西省卫生厅中医药科研基金

摘　　要：目的:探讨补肾健脾法对原因不明复发性流产(URSA)患者外周血CD4+CD25+调节性T细胞(Tr)的影响。方法:对南昌大学第四附属医院及江西省中医院2008年1月至2009年12月就诊的20例URSA患者,采用双荧光标记流式细胞分析技术,检测补肾健脾治疗前后外周血CD4+CD25+Tr细胞表达频率的变化。结果:补肾健脾中药治疗后,URSA患者外周血中CD4+CD25brightTr较治疗前明显增加(P<0.05),CD4+CD25dimTr表达频率较治疗前降低(P<0.05),中药安胎妊娠成功妇女CD4+CD25brightTr显著高于妊娠失败者。结论:补肾健脾可提高外周血CD4+CD25brightTr数量,能够提高机体保护性免疫功能,维持机体内环境稳定,为URSA的有效治疗方法。Objective To investigate the influence of nourishing kidney and strengthing spleen on peripheral blood CD4 ＋ and CD25 ＋ T regulatory cells in unexplained recurrent spontaneous abortion （URSA）. Methods Twenty patients who had had clinical visit from January 2008 to December 2009 were enrolled in this study. The percentage of D4 ＋and CD25 ＋ T cells in peripheral blood were measured by double-label flow cytometry before and after therapy with nourishing kidney and strengthing spleen. Results After nourishing kidney and strengthing spleen, the percentage of CD4＋ and CD25bright T cells increased significantly （P 0.05）, while CD4＋ and CD25dim T cells decreased markedly（P 0.05）. The percentage of CD4＋ and CD25bright T cells was significantly higher in the successful pregnant women than in those failed in pregnancy after using traditional Chinese medicine. Conclusions Therapy with nourishing kidney and strengthing spleen can enhance the percentage of CD4＋ and CD25bright T cells in peripheral blood, increase immune function, and maintain the inner environment of the body. It is an effective therapy for unexplained recurrent spontaneous abortion.

关 键 词：流产 CD4+CD25+调节性T细胞 补肾健脾 

分 类 号：R271.9[医药卫生—中西医结合]