运动及补充白藜芦醇对小鼠骨骼肌线粒体的影响  

Effects of Exercise and Resveratrol on the Changes of Skeletal Muscle Mitochondria of Mouse

作　　者：闻剑飞[1,2] 王海涛[1] 于树宏[3] 刘玉倩[1] 袁克星[1] 金凤霞[1] 

机构地区：[1]河北师范大学体育学院,石家庄050016 [2]合肥师范学院体育教学部,合肥230016 [3]河北医科大学药学院,石家庄050017

出　　处：《天津体育学院学报》2010年第4期344-347,共4页Journal of Tianjin University of Sport

基　　金：河北省教育厅基金项目(项目编号:Z2008119);河北师范大学青年基金项目(项目编号:L2007Q20)

摘　　要：目的:观察补充白藜芦醇对运动引起的骨骼肌线粒体形态学变化及线粒体Ca2+变化的影响。方法:70只雄性昆明种小鼠随机分为安静对照组(control group,CG)、运动对照组(exercise group,EG)和运动+白藜芦醇组(exercise+resveratrol group,ER),EG组、ER组又分为3个亚组,运动后即刻组、运动后24 h组、运动后48 h组,每组10只。ER组每日给予白藜芦醇10 mg/kg灌胃,与EG组同时进行4周的跑台训练,训练结束后进行一次坡度为0,速度为18 m/min,持续90 min的跑台运动。运动结束后按组别取材,电镜观察肌骨骼肌线粒体形态学变化;测定肌组织SOD活性、MDA含量、线粒体Ca2+浓度。结果:电镜结果表明,EG组小鼠运动后骨骼肌线粒体出现了肿胀、空化、髓样化等现象;ER组小鼠运动后骨骼肌线粒体虽也出现相似病变,但在运动后48 h线粒体病变明显减轻;ER组骨骼肌中SOD活性显著升高、MDA含明显降低,与EG组相比,差异具有显著性(P<0.05);EG组运动后各时刻线粒体Ca2+含量明显增加,48 h后仍末恢复(P<0.05);ER组运动后虽线粒体Ca2+含量也明显增加,但运动后48 h已明显降低,差异具有显著性(P<0.05)。结论:一次长时间剧烈运动可引起骨骼肌线粒体发生损伤性变化;补充白藜芦醇可以提高骨骼肌抗氧化能力,降低脂质过氧化反应,调节线粒体Ca2+变化,对线粒体的结构与功能具有一定的保护作用。Objective：To observe the effect of resveratrol on exercise-induced skeletal muscle mitochondrial morphology and mitochondrial Ca2＋ change.Methods：70 male Kunming mice were randomly divided into three groups：control group（CG）,exercise group（EG）and exercise＋resveratrol group（ER）,and EG group and ER group Were divided into three sub-groups by post-exercise 0 hour,24 hours,48 hours,which every group has 10 mice.The mice of group ER were given resveratrol with 10 mg（/kg·d）,and taken protocol of treadmill training for 4 weeks,then had a gradient of 0 and a speed of 18m/ min,continued 90 min of treadmill exercise as did the mice of group EG.Mice were slay respectively.The histological change of mitochondrial were observed by electron microscope.The activity of muscle SOD and MDA,concentration of muscle mitochondria Ca2＋ were measured using Ultraviolet Spectrometer.Results：The electron microscope results showed that EG appeared swelling,cavitation,myeloid and some of the mitochondria were irregularly arranged,ER mice Skeletal muscle mitochondria with ridges,membrane fusion,fuzzy unclear after exercise,and the injury level of ER was less than EG.skeletal muscle of ER group SOD activity was significantly increased,MDA with significantly decrease compared with the EG group,a significant difference（P0.05）.Compare with CG,the concentration of mitochondria Ca2＋ was significantly increased in EG,especially post-exercise 24 h（P0.05）.The concentration of mitochondria Ca2＋ of ER was significantly lower than that of EG（P0.05）.Conclusion：After the long time treadmill exercise,the muscle mitochondrial presents the obvious damage change,supplement resveratrol can improve mice antioxidant capacity,lower lipid peroxidation,and regulation of muscle mitochondrial Ca2＋ concentration,so resveratrol could have a certain protective effect on structure and function of mitochondria.

关 键 词：白藜芦醇 骨骼肌 线粒体 

分 类 号：G804.7[文化科学—运动人体科学]