槲皮素抗人脐静脉内皮细胞氧化损伤的作用研究  

Protective Effect of Querceti on the Injury of Vascular Endothelial Cell Induced by Hydrogen Peroxide

作　　者：李东娟[1] 万云云[2] 

机构地区：[1]泰山医学院病理学教研室,山东泰安271000 [2]泰安卫生学校,山东泰安271000

出　　处：《泰山医学院学报》2010年第5期337-340,共4页Journal of Taishan Medical College

摘　　要：目的观察槲皮素(quercetin,Que)对人脐静脉内皮细胞(human umbilical vein endothelial cells,HUVECs)过氧化氢损伤的保护作用及可能机制。方法用体外培养的HUVECs传代后进行实验,实验分为3组:对照组常规培养;过氧化氢损伤组;药物干预组加入Que低、中、高浓度组(62.5、125、250 umol/L预处理)。用MTT法观察Que对过氧化氢损伤的内皮细胞活性的影响,各组均测定细胞中超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛((malondialdehyde,MDA)和乳酸脱氢酶(lactate dehydrogense,LDH)的活性,TUNEL法检测细胞凋亡率。结果 Que使过氧化氢损伤的内皮细胞的脂质过氧化物MDA生成减少,LDH漏出液减少,SOD活力增加;过氧化氢损伤可以诱导内皮细胞凋亡,Que可显著减少过氧化氢诱导的内皮细胞的凋亡。结论 Que处理对过氧化氢所致的内皮细胞的损伤有保护作用,机制可能通过抗脂质过氧化,对氧自由基的清除,以及抗细胞凋亡有关。Objective：To investigate the protective effect of querceti on the injury of human umbilical vein endothelial cells（HUVECs） induced by hydrogen peroxide and its corresponding mechanisms.METHODS： The endothelial cell strain from human umbilical vein was cultured and a model of VEC injured by hydrogen peroxide（H2O2） was established.This study was conducted as follows： normal control group,H2O2 injury group,querceti protective groups（62.5、125、250 umol /L）.Cell viability was tested by using MTT.The levels of malondialdehyde（MDA）,superoxide dismutase（SOD） and lactate dehydrogense（LDH） activity were measured with corresponding kit.HUVECs apoptotic rate was detected by TUNEL.RESULTS： Querceti could inhibit the hydrogen peroxide induced VEC reduction,suppress LDH activity,reduce theMDA production,and enthance SOD activity under hydrogen peroxide.Typical apoptotic cells were detected by TUNEL.CONCLUSION：These results demonstrated that querceti can protect the VECs from H2O2 induced injury,and the possible mechanism may refer to its effect of anti-apoptosis,anti-lipid peroxidation and SOD activity enhancing.

关 键 词：人脐静脉内皮细胞 槲皮素 过氧化氢 氧化应激 细胞凋亡 

分 类 号：R285[医药卫生—中药学]