植酸酶高产菌株的诱变选育  

Mutation Breeding of Phytase High-Yielding Strain

作　　者：王志红[1] 董晓芳[1] 佟建明[1] 张国庆[1] 张琪[1] 许尚忠[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所动物营养学国家重点实验室,北京100193

出　　处：《微生物学杂志》2010年第4期83-87,共5页Journal of Microbiology

基　　金：国家"十一五"科技支撑计划项目(2006BAD12B05);动物营养学国家重点实验室自主研究课题(2004DA125184G0811)

摘　　要：无花果曲霉是一种可以产植酸酶的菌株，其代谢产物植酸酶可以将有机植酸磷降解为无机磷。以此菌株为出发菌株，确定了它的最适pH值为1．3—1．4，最适温度为55-60℃。同时，为获得高酶活的突变株，进行亚硝基胍和紫外线处理，经初筛得到99株高效突变株，再经复筛和传代试验，得到1株植酸酶活性是出发菌株2．47倍的突变株NTG-23。Aspergillus ficuum is a strain that could produce phytase. Its metabolites could degrade organic phosphate into inorganic phosphate. It was taken as a starting strain to begin with the study. And confirmed pH 1.3 ~ 1.4 was the most suitable value and optimum temperature was 55 -60 ℃ respectively. In order to obtain mutant strain with high enzymatic activity, UV and NTG treatment were taken and obtained 99 mutant strains with highly effective through primary screening. One strain （NTG-23） was obtained through secondary screening and generation passing experiment, its enzymatic activity was 2.47 times of the starting strain.

关 键 词：无花果曲霉 植酸酶 诱变 亚硝基胍 紫外线 

分 类 号：Q939.9[生物学—微生物学]