生防木霉菌β-1,3-葡聚糖酶活性研究  

β-1,3-Glucanase Activity from Biocontrol Trichoderma Strains

作　　者：李世贵[1] 顾金刚[1] 姜瑞波[1] 牛永春[1] 

机构地区：[1]中国农业科学院农业资源与农业区划研究所中国农业微生物菌种保藏管理中心,北京100081

出　　处：《微生物学杂志》2010年第4期88-91,共4页Journal of Microbiology

基　　金：国家科技基础条件平台建设项目(2005DKA21201)

摘　　要：通过采取还原糖法对生防木霉菌真菌2号和真菌4号菌株在不同发酵时间产β-1,3-葡聚糖酶活性进行测定,对其产β-1,3-葡聚糖酶特性进行初步研究。结果表明,同一菌株在不同发酵时间β-1,3-葡聚糖酶活性大小变化的趋势大致相同,并均在培养72 h时β-1,3-葡聚糖酶活性达到最大,在相同发酵时间,真菌2号菌株比真菌4号菌株的β-1,3-葡聚糖酶活性要高。Method of reduetive sugar was adopted to carry out the determination of β-1,3-glucanase activity of biocon- trol Trichoderma strains No. 2 and No. 4 at different fermentation times and preliminarily studied the properties of 6-1, 3-glucanase they produced. The result indicated that the change tendency of β-1,3-glucanase activity of the identical strain was approximately the same at the different fermentation time. And the β-1,3-glucanase activity reached the highest at 72 hours of culture. At same fermentation time, the β-1,3-glucanase activity of the strain No. 2 was higher than that of the strain No. 4.

关 键 词：β-1  3-葡聚糖酶 还原糖法 木霉菌 生物防治 

分 类 号：Q939.9[生物学—微生物学]