非小细胞肺癌中FHIT的表达及DNA含量和细胞增殖活性的检测研究  

THE EXPRESSION OF FHIT AND DETERMINATION OF DNA INDEX AND PROLIFERATION INDER IN NON-SMAIL LUNG CANCER

作　　者：郭妍妍[1] 李秀霞[2] 

机构地区：[1]内蒙古医学院附属医院儿科,内蒙古呼和浩特010050 [2]内蒙古医学院病理教研室

出　　处：《内蒙古医学院学报》2010年第4期363-366,共4页Acta Academiae Medicinae Neimongol

摘　　要：目的:研究非小细胞肺癌(non-small cell lung cancer,NSCLC)中FHIT的表达和检测DI、PI与肺癌发生发展的关系。方法:采用免疫组化(S-P法)和流式细胞术(flow cytometry FCM)检测法。结果:FHIT在正常支气管黏膜及NSCLC中的表达有显著性差异(P<0.05),其表达与分化程度,吸烟有显著性差异(P<0.01,P<0.05),与性别,年龄,TNM分期及分型无关。吸烟与鳞癌呈正相关。NSCLC中DI、PI比正常支气管黏膜明显增高(P<0.05,P<0.05),DI、PI与肺癌分型、分期、分化程度均无相关性(P>0.05)。结论:1.FHIT是非小细胞肺癌发生发展分子机制之一。2.FHIT可能与烟草致癌有相关性。3.DI及PI是检测非小细胞肺癌早期发生发展的恒定指标。Objective：To investigate the role of FHIT,DI and PI in NSCLC.Methods：Detect the expression of FHIT,DI and PI and by immunochemical（S-P）and flow cytometry.Results：The postive expression rates of FHIT are significantly lower than those in nomal lung tisses（P〈0.05）.The expression rates of FHIT are correlated with degree of differentiation of NSCLC and smoking history（P〈0.01,P〈0.05）.There are no relativity with TNM stage、age、sex and histology（P〉0.05）.There are the relativity between smoking history and squamous cell.DI,PI are significantly higher in NSCLC than those in normal lung tissue（P〈0.01,P〈0.05）.DI and PI have no correlation with other items（P〉0.05）.Conclusion：（1）FHIT is one of events in NSCLC.（2）FHIT may be relativited with NSCLC produced by smoking.（3）DI、PI are items during primary NSCLC.

关 键 词：脆弱性组氨酸三联体 DNA含量 细胞增殖活性 非小细胞肺癌 

分 类 号：R711.74[医药卫生—妇产科学] R737.33[医药卫生—临床医学]