反相高效液相色谱法测定蒙药复方森登-4中栀子苷的含量  

THE CONTENT OF GENIPOSIDE IN MONGOLIAN MEDICINE COMPOUND senden-4 BY RP-HPLC

作　　者：武娜[1] 董玉[1] 贾鑫[1] 

机构地区：[1]内蒙古医学院药学院,内蒙古呼和浩特010059

出　　处：《内蒙古医学院学报》2010年第4期387-389,共3页Acta Academiae Medicinae Neimongol

基　　金：内蒙古自治区自然科学基金项目(2009Zd18);内蒙古自治区重大科技项目(2009)

摘　　要：目的：建立蒙药复方森登-4中栀子苷的含量测定方法。方法：采用反相高效液相色谱法,色谱柱为Hypersil ODS 25um（4.6mm.250um）。流动相为甲醇-0.4%乙酸溶液（20∶80）,检测波长为238nm,流速为1.0mL·omin^-1。结果：栀子苷在0.001215-0.01215mg范围内具有良好的线性关系,r=0.9999,平均加样回收率为99.39%,RSD=2.48%。结论：本方法快速,准确,重复性好,可用于蒙药复方森登-4中栀子苷的含量测定。Objective：To establish a method to determine the content of Geniposide in sendeng-4.Methods：The column was Hypersil ODS 25um（4.6mm·250um）,mobile phase was methanol-0.4% acetic acid solution（20∶80）,wavelength was 238nm,flow rate was 1.0mL·min^-1by RP-HPLC rndthed.Results：Geniposide-4 had a good linear relationship in the range of 0.001215～0.01215mg,r=0.9999,the average recovery rate is 99.39%,RSD =2.48%.Conclusion：This method is fast,accurate,repeat better and can be used to determine the content of Geniposide in Mongolian medicine Senden-4 with HPLC.

关 键 词：HPLC 蒙药复方森登-4 栀子苷 

分 类 号：R962[医药卫生—药理学]