尿微量白蛋白胶乳凝集抑制试验药盒的研制及其肾脏损害早期诊断价值的评估  

The development and application of latex agglutination inhibition kit for urinary microalbumin

作　　者：徐新女[1] 刘萍[1] 韩亚娟[1] 张文[1] 吴胜群[1] 

机构地区：[1]天津市第一中心医院

出　　处：《中国危重病急救医学》1999年第4期206-209,共4页Chinese Critical Care Medicine

基　　金：天津市卫生局青年课题

摘　　要：目的：探讨自制免疫胶乳凝集抑制试验药盒半定量检测尿中微量白蛋白的准确性及其对肾脏损害的早期诊断价值。方法：用０．２μｍ微粒胶乳制备出尿微量白蛋白胶乳凝集抑制试验药盒，检测４１例健康人、７３例糖尿病患者和５７例脑血管病患者的随意尿标本中尿微量白蛋白，并与国产酶联免疫吸附（ＥＬＩＳＡ）和进口ＭｉｃｒａｌＴｅｓｔⅡ试剂的测定结果进行比较。结果：胶乳法、ＥＬＩＳＡ法和ＭｉｃｒａｌＴｅｓｔⅡ法对健康组尿白蛋白测定的阳性率分别为４．８８％、７．３２％和７．３２％，对糖尿病组分别为２４．６６％、２６．０３％和２７．４０％，对脑血管病组分别为２２．８１％、２４．５６％和２６．３２％；胶乳法与ＥＬＩＳＡ和ＭｉｃｒａｌＴｅｓｔⅡ试剂测定一致率分别为９８．２４％和９７．０８％。结论：采用自制免疫胶乳凝集抑制试验药盒测定尿微量白蛋白方法简便、快速、价廉，对糖尿病肾病、肾小球肾炎等疾病的早期诊断和预后判断具有较高的应用价值。Objective:To develop a latex agglutination inhibition kit for detection of urinary microalbumin,and to assess its clinical value in early diagnosis of diabetic nephropathy.Methods:A latex agglutination inhibition kit was developed with latex particles of 0 2 μm in diameter for detection of urinary microalbumin.Using such new kit,urinary microalbumin contents were determined in 41 healthy volunteers,73 patients with diabetes,and 57 patients with cerebral diseases.Results:By the use of latex agglutination inhibition,enzyme linked immunosorbent assay(ELISA) and imported MicralTest Ⅱ kits,the positive rates of urinary microalbumin respectively were 4 88%,7 32% and 7 32% in healthy group,24 66%,26 03% and 27 40% in diabetic patients,and 22 81%,24 56% and 26 32% in cerebral patients.Also,the coincidence rate between latex agglutination inhibition and ELISA or MicralTest Ⅱ kit was 98 24% or 97 08%,respectively.Conclusions:The latex agglutination inhibition test is a simple,rapid,inexpensive method to detect urinary microalbumin.It is of importance in early diagnosis and prediction of diabetic nephropathy and glomerulonephritis.

关 键 词：糖尿病肾病 尿微量白蛋白 诊断 ELISA 

分 类 号：R587.204[医药卫生—内分泌]