Construction and Characterization of Grass Carp (Ctenopharyngodon idellus) Fosmid Library  

作　　者：JIA Zhen-hu LIN Chang-you YANG Tian-yao JIANG Yi-nan XIA Chun 

机构地区：[1]Department of Microbiology and Immunology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, P.R.China [2]College of Life Science, Shanxi Normal University, Linfen 041004, P.R. China

出　　处：《Agricultural Sciences in China》2010年第9期1347-1352,共6页中国农业科学（英文版）

基　　金：supported by the National Natural Science Foundation of China (30371 098)

摘　　要：Grass carp （Ctenopharyngodon idellus） genomic fosmid library cotaining 129 014 clones was constructed and characterized from one diploid grass carp. The average insert size of the fosmid library was determined to be 35 kb by pulsed field gel electrophoresis, which is 4.1-fold coverage of the grass carp genome. To demonstrate the probability of picking the functional genes from the library, eleven functional genes were screened by three-dimensional PCR technique. The number of positive clones of these genes was from 1 to 6. So, this library may screen any useful genes from grass carp. This grass carp genome fosmid library will be integrated in the presently ongoing efforts to determine the sequence of the grass carp genome.Grass carp （Ctenopharyngodon idellus） genomic fosmid library cotaining 129 014 clones was constructed and characterized from one diploid grass carp. The average insert size of the fosmid library was determined to be 35 kb by pulsed field gel electrophoresis, which is 4.1-fold coverage of the grass carp genome. To demonstrate the probability of picking the functional genes from the library, eleven functional genes were screened by three-dimensional PCR technique. The number of positive clones of these genes was from 1 to 6. So, this library may screen any useful genes from grass carp. This grass carp genome fosmid library will be integrated in the presently ongoing efforts to determine the sequence of the grass carp genome.

关 键 词：grass carp genome fosmid library three-dimensional PCR 

分 类 号：Q987[生物学—遗传学] S965.112[生物学—人类学]