从红豆杉树皮浸膏中提取紫杉醇初分离工艺的研究  

Studies on Initial Separation of Paclitaxel from the Taxus Yunnanensis Extract

作　　者：张志强[1] 田桂莲[1] 冯小黎[1] 韦新桂[2] 苏志国[2] 

机构地区：[1]大连理工大学生化工程研究所,116012 [2]中国科学院化工冶金研究所生化工程国家重点实验室

出　　处：《中国生化药物杂志》1999年第2期58-61,共4页Chinese Journal of Biochemical Pharmaceutics

基　　金：国家九五攻关项目

摘　　要：从红豆杉树皮浸膏中高效提取紫杉醇。方法；采用液－液萃取、固相萃取、硅胶柱层析、氧化铝柱层析、薄层层析五种分离方法对紫杉醇进行了初分离。结果：优化的紫杉醇初分离方案为红豆杉树皮提取物先经氧化铝柱，再上固相萃取柱进行分离提取。结论：该工艺分离所得紫杉醇浓度可达４０％以上，紫杉醇的回收率超过１０％。Purpose: The aim was to separate paclitaxel from the Taxus Yunnanensis extract efficiently. Methods: Liquid-liquid partition, solid-phase separation extraction. silica-gel chromatography, Al2O3 chromatography and thin-layer chromatography were performed and compared for separation of paclitaxel. Results: An optimum strategy was found to combine Al2O3 chromatography with solid-phase separaltion extraction. Conclusion: The final purity of paclitaxel reached .40％ after one step Al2O3 chromatography followed by a solid-phase extraction. The recovery of paclitaxel was over 100％.

关 键 词：紫杉醇 初分离 层析 红豆杉树皮浸膏 

分 类 号：TQ461[化学工程—制药化工]