美洲钩虫及十二指肠钩虫细胞色素C氧化酶1基因测序  

SEQUENCING OF CYTOCHROME C OXIDASE 1 GENE OF ANCYLOSTOMA DUODENALE AND NECATOR AMERICANUS *

作　　者：李铁华[1] 詹斌[1] JOHN M HAWDON 龚昕[1] 肖树华[1] 单强[1] 冯正[1] PETER J HOTEZ 

机构地区：[1]中国预防医学科学院寄生虫病研究所 [2]美国耶鲁大学医学院公共卫生与流行病学系

出　　处：《中国寄生虫学与寄生虫病杂志》1999年第2期81-83,共3页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：美国热带医学研究中心(TMRC)资助;纽约中国医学董事会INC及NIH美国心脏协会资助

摘　　要：目的：比较美洲钩虫及十二指肠钩虫线粒体细胞色素Ｃ氧化酶１（ＣＯ１）基因序列，确定两种钩虫间ＣＯ１基因差异。方法：现场采集钩虫成虫样本，蛋白酶Ｋ消化抽提线粒体基因组ＤＮＡ，ＰＣＲ方法扩增ＣＯ１基因，ＤＮＡ测序及分析。结果：ＰＣＲ扩增获得约７００ｂｐ美洲钩虫及十二指肠钩虫ＣＯ１基因片段，其序列比较显示两种钩虫ＣＯ１基因同源性达８９．７％，存在特定的核苷酸差异。结论：ＣＯ１基因可作为鉴别两种人体钩虫虫种标记性基因。AIM: To identify the genetic diversity between Ancylostoma duodenale and Necator americanus. METHODS : Mitochondrial cytochrome C oxidase subunit 1 (CO1) gene was amplified from genomic DNA of human hookworms collected from infected patients in Hejiang County, Sichuan Province , and the purified PCR products were directly sequenced by using Licor auto sequencer. RESULTS: The PCR products were about 700 bp. Alignment of CO1 gene fragment sequences showed 89 7% similarity between Ancylostoma duodenale and Necator americanus, but still certain nucleotide variations (10 3%) existed. CONCLUSION: CO1 gene sequence can be used as a marker to identify the two species of human hookworms.

关 键 词：钩虫 DNA序列 美洲钩虫 十二指肠钩虫 CO1 

分 类 号：R383.13[医药卫生—医学寄生虫学]