瑞香素的抗溶血和抗膜脂质过氧化作用  

ANTI HEMOLYTIC AND ANTI MEMBRANOUS LIPID PEROXIDATION EFFECTS OF DAPHNETIN *

作　　者：倪奕昌[1,2] 徐月琴[1,2] 王鸣杰[1,2] 刘云光[1,2] 

机构地区：[1]中国预防医学科学院寄生虫病研究所 [2]世界卫生组织疟疾,血吸虫病和丝虫病合作中心

出　　处：《中国寄生虫学与寄生虫病杂志》1999年第2期87-89,共3页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：中国预防医学科学院科研基金

摘　　要：目的：研究瑞香素的抗溶血与抗红细胞膜脂质过氧化作用。方法：用常规的溶血毒性体外检测法及高效液相层析定量测定脂质过氧化产物法，检测不同浓度瑞香素对伯氨喹（ＰＱ）溶血毒代谢产物５，６－二羟基８－氨基喹啉（ＡＱＤ）诱发溶血与红细胞膜脂质过氧化的抑制率。结果：１０至８０μｍｏｌ／Ｌ瑞香素对ＡＱＤ溶血毒性与红细胞膜脂质过氧化的抑制率分别为３３．０％－６９．２％和１１．９％－５８．２％，且呈一定的剂量－反应关系。结论：瑞香素具有抗溶血与抗红细胞膜脂质过氧化作用。AIM: To investigate the anti hemolytic and anti membranous lipid peroxidation effects of daphnetin. METHODS: The inhibition rates of daphnetin on AQD induced hemolysis and erythrocytic membranous lipid peroxidation were determined by routine in vitro assay for detecting hemolytic toxicity and by HPLC for detecting membranous lipid peroxidation products. RESULTS: Daphnetin at a range of 10-80 μmol/L inhibited dose dependently AQD induced hemolysis and lipid peroxidation by 33 0%-69 2% and 11 9%-58 2%, respectively. CONCLUSION: Daphnetin has anti hemolytic and anti erythrocytic membranous lipid peroxidation effects.

关 键 词：瑞香素 抗溶血 抗膜脂质过氧化 AQD 

分 类 号：R285.5[医药卫生—中药学]