抗微小隐孢子虫单克隆抗体免疫保护作用的研究  

STUDIES ON IMMUNOPROTECTION OF MONOCLONAL ANTIBODIES AGAINST CRYPTOSPORIDIUM PARVUM

作　　者：宗海红[1,2,3] 李为明[1,2,3] 张雪 俞树荣[1,2,3] 白班俊[1,2,3] 

机构地区：[1]第三军医大学附属新桥医院儿科 [2]第三军医大学微生物教研室 [3]解放军304医院

出　　处：《中国寄生虫学与寄生虫病杂志》1999年第2期101-105,共5页Chinese Journal of Parasitology and Parasitic Diseases

摘　　要：目的：探讨抗隐孢子虫单克隆抗体（ＭｃＡｂ）的免疫保护作用。方法：在细胞培养的基础上进行体外中和试验，筛选具有保护作用的ＭｃＡｂ，并通过大鼠及ＭＤＣＫ细胞接种隐孢子虫子孢子（ＣＰＳ）进行动物实验和受染ＭＤＣＫ细胞透射电镜观察证实。结果：Ｚ３Ｄ２可显著降低ＣＰＳ在大鼠肠粘膜表面定植的隐孢子虫虫体及卵囊数。可使隐孢子虫各发育期的虫体减少，使ＭＤＣＫ细胞的超微结构受损减轻。结论∶ＭｃＡｂＺ３Ｄ２具有良好的保护作用。AIM: To explore the immune protection of the monoclonal antibody (McAb) againstCryptosporidium parvum.METHODS: On the basis of Madin Darby canine kidney (MDCK) cell culture, the immunoprotection of McAb against C.parvum was screened by means of neutralization test in vitro, and verified by rat model and transmission electron microscopy of infected MDCK cells. RESULTS: The number of C.parvum sporozoites recovered on the surface of rat intestinal epithelial cells and the mean output of oocyst were significantly reduced by McAb Z3D2 (P<0 001, P<0 05). The decreased Cryptosporidium at each development phase within infected MDCK cells and less damage of cell ultrastructure caused by Cryptosporidium were found in Z3D2 treated group. CONCLUSION: McAb Z3D2 possesses highly effective protection against C.parvum.

关 键 词：微小隐孢子虫 MDCK细胞 单克隆抗体 免疫预防 

分 类 号：R531.5[医药卫生—内科学]