微波辐射ELISA诊断日本血吸虫病的初步研究  

PRELIMINARY STUDIES ON MICROWAVE IRRADIATION ELISA FOR DIAGNOSIS OF SCHISTOSOMIASIS JAPONICA

作　　者：顾启章[1,2] 张宏莹[1,2] 朱善济 徐文娟[1,2] 

机构地区：[1]杭州医学高等专科学校 [2]浙江省人民医院病理科

出　　处：《中国寄生虫学与寄生虫病杂志》1999年第2期115-116,共2页Chinese Journal of Parasitology and Parasitic Diseases

摘　　要：目的：探索高效快速的血吸虫病诊断方法。方法：用微波辐射ＥＬＩＳＡ和快速ＥＬＩＳＡ同步检测各期日本血吸虫病患者血清１１８份，健康人血清６１份，并殖吸虫病犬血清１２份。结果：两种方法的血吸虫病患者血清抗体阳性检出率分别为８８．１％（１０４／１１８）和９１．５％（１０８／１１８），无显著性差异。健康人血清抗体假阳性分别为２份（３．３％）和１份（１．６％），亦无显著性差异；交叉反应均无假阳性。结论：微波辐射ＥＬＩＳＡ具有与快速ＥＬＩＳＡ类似的敏感性和特异性，但前者具有快速获得检测结果的优点。AIM: To explore a fast and highly efficient method for the diagnosis of schistosomiasis japonica. METHODS: Using microwave irradiation ELISA(MWI ELISA)and fast ELISA to detect specific antibodies in sera from 118 cases with schistosomiasis japonica, 61 healthy individuals and 12 paragonimiasis cases. RESULTS: The positive rates of schistosomiasis cases were 88 1% (104/118) by MWI ELISA and 91 5% (108/118) by fast ELISA, respectively (χ 2=0 74, P>0 05). The false positive reaction of healthy individuals was found in 2 cases(3 3%) by MWI ELISA and 1 case (1 6%) by fast ELISA, respectively (χ 2=0 34, P>0 05). No false positive reaction was found in paragonimiasis cases. CONCLUSION: The sensitivity and specificity of the two tests were similar, however, MWI ELISA was faster than fast ELISA.

关 键 词：血吸虫病 血清学诊断 抗体检测 ELISA 

分 类 号：R532.210.4[医药卫生—内科学]