弓形虫感染鼠血清中弓形虫循环抗原的检测  

DETECTON OF CIRCULATING ANTIGEN IN SERA FROM MICE INFECTED WITH TOXOPLASMA TACHYZOITES

作　　者：金武官[1,2] 李云珠[1,2] 俞善昌[1,2] 杨惠珍[1,2] 钱宗立[1,2] 

机构地区：[1]上海第二医科大学附属瑞金医院儿科 [2]上海第二医科大学寄生虫学教研室

出　　处：《中国寄生虫学与寄生虫病杂志》1999年第2期117-118,共2页Chinese Journal of Parasitology and Parasitic Diseases

摘　　要：目的：探索弓形虫感染早期诊断方法。方法：用快速ＥＬＩＳＡ检测弓形虫感染鼠血清中弓形虫循环抗原。结果：在轻、中、重３个感染组，分别于感染后ｄ４、ｄ４及ｄ３检测到弓形虫循环抗原。结论：感染虫体数量越多则弓形虫循环抗原测出时间越早，ＯＤ值亦越高。检测弓形虫循环抗原具有早期诊断价值。AIM: To explore an assay method for the early diagnosis of Toxoplasma infection. METHODS: Serum samples collected from three groups of mice infected experimentally with different doses of Toxoplasma trophozoites were detected for the presence of Toxoplasma circulating antigen by using fast ELISA.RESULTS: Toxoplasma circulating antigen was detected on days 4, 4 and 3after infection in light, moderate and heavy infection groups, respectively. CONCLUSION: The level of circulating antigen was in parallel with the duration of infection. The determination of circulating antigen is useful in the early diagnosis of Toxoplasma infection.

关 键 词：弓形虫 循环抗原 鼠血清 弓形体病 

分 类 号：R531.8[医药卫生—内科学]