玳玳果及其总黄酮有效部位的HPLC指纹图谱的研究  

Studies on HPLC fingerprints of medical material and effective parts containing total flavonoids of Citrus Aurantium L.var daidai Tanaka fruits

作　　者：刘永静[1] 陈丹[1] 于丽丽[1] 邱红鑫[1] 黄群[1] 

机构地区：[1]福建中医药大学药学系,福州350108

出　　处：《中华中医药杂志》2010年第9期1491-1495,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：福建省自然科学基金项目(No.2008J0095);福建省教育厅项目(No.JA05283);福建省科技计划重点课题项目(No.2010Y0030)~~

摘　　要：目的:建立玳玳果药材及其总黄酮有效部位的HPLC指纹图谱,为科学评价其质量控制提供有效的方法。方法:运用HPLC对6批玳玳果及其总黄酮有效部位进行指纹图谱的分析和方法学考察,流动相采用0.05%磷酸水-0.05%磷酸乙腈梯度洗脱,250-4LichrocartC18色谱柱(250mm×4.6mm,5μm),检测波长284nm,流速1.0mL/min,柱温25℃。结果:建立了玳玳果药材及玳玳果总黄酮有效部位的HPLC指纹图谱共有模式,各批玳玳果药材及其总黄酮有效部位的相似度均>0.900,玳玳果药材指纹图谱与玳玳果总黄酮有效部位指纹图谱具有良好的相关性。结论:所建立的方法精密度、稳定性及重复性良好,特征性及专属性强,可作为有效控制玳玳果药材及其总黄酮有效部位质量的方法。Objective：To establish sensitive and specific HPLC fingerprints for the quality control of medical material and the effective parts containing total flavonoids of Citrus Aurantium L.var daidai Tanaka fruits.Methods：The HPLC was used to establish the fingerprints and the HPLC analysis was performed on a 250-4 Lichrocart C18 colmnn（250mm×4.6mm,5μm）with the mixture of solvent A（0.05%phosphoric acid）and solvent B[acetonitrile-phosphoric acid（95：5）]in gradient mode at a flow rate of 1.0 mL？min-1.The detection wavelength was set at 284nm.The column temperature was set at 25℃and the injection volume was 20μL.Results：The chromatographic fingerprints of the medicine from different patches was0.900.Comparison of the HPLC profiles of the total flavonoids material and the effective parts of the total flavonoids was established and the similarity with the corresponding Citrus Aurantium L.var daidai Tanaka fruits indicated that they were closely related to each other.Conclusion：The HPLC fingerprints of the medicine material and the effective parts of the total flavonoids with high specificity and can be used to control its quality.

关 键 词：玳玳果 总黄酮 有效部位 HPLC 指纹图谱 

分 类 号：R284[医药卫生—中药学]