补骨脂汤对血管性痴呆大鼠海马内雌激素受体-β、NMDAR2B基因表达的影响  

Effects of Buguozhi Decoction on gene expression of ER-β,NR2B in hippocampus of vascular dementia model rats

作　　者：郑里翔[1] 邓科穗[2] 乔玉丹[1] 施旻[1] 崔志远[1] 

机构地区：[1]江西中医学院基础医学院,南昌330004 [2]江西中医学院附属医院,南昌330004

出　　处：《中华中医药杂志》2010年第9期1496-1498,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：江西省自然科学基金(No.0610159);江西省教育厅项目(No.GJJ09281);江西省卫生厅项目(No.2008Z0028)~~

摘　　要：目的:补骨脂汤改善血管性痴呆大鼠学习记忆的作用。方法:通过双侧颈动脉结扎制作血管性痴呆模型,用RT-PCR及免疫组化方法测定海马内雌激素受体β(ER-β)、NMDAR2B(NR2B)受体基因的表达产物。结果:补骨脂汤组ER-β、NR2B基因表达的mRNA量明显高于模型组及银杏片组(P<0.05);补骨脂汤组大鼠海马CA3区的阳性细胞数均高于模型组、银杏片组(P<0.05)。结论:补骨脂汤能改善血管性痴呆大鼠的学习记忆,可能的作用途径是提高ER-β受体、NR2B受体的基因表达水平,从而提高学习记忆。Objective：To investigate the mechanism of Buguozhi Decoction in improving learning-memory of vascular dementia model rats.Methods：The rat models with VaD were established by bilateral occlusion of both common carotid arteries（2 一V0）.The gene expressions of ER-β,NR2B were evaluated by RT-PCR and immunohistochemistry.Results：Buguozhi Decoction can significantly improve mRNA and protien pruducts of ER-β,NR2B gene in hippocampus of the vascular dementia model rats,compared with the model group（P0.05）.Conclusion：The mechanism of Buguozhi Decoction in improving the learningmemory of the vascular dementia model rats maybe to increase the expression of ER-βand NR2B gene.

关 键 词：补骨脂汤 血管性痴呆 雌激素受体Β NR2B受体基因 学习记忆 

分 类 号：R285.5[医药卫生—中药学]