超声量化技术在大鼠脂肪肝模型中应用的初步研究  

Preliminary study on application of the quantitative ultrasonic diagnostic technique in the study on a rat model of fatty liver disease

作　　者：徐旭[1] 席文恭[1] 王维亭[1] 赵专友[1] 汤立达[1] 

机构地区：[1]天津药物研究院天津药代动力学与药效动力学省部共建国家重点实验室,天津300193

出　　处：《药物评价研究》2010年第4期263-266,共4页Drug Evaluation Research

基　　金：国家新药重大创制项目(2009zx09301-008)

摘　　要：目的:探讨超声量化技术评价大鼠肝脏脂肪变的价值。方法:大鼠ig酒精高脂乳液12周,制备脂肪肝模型。测量正常大鼠及模型大鼠肝脏二维声像图的灰度值,检测肝组织三酰甘油(TG)、总胆固醇(TC),并将肝脏组织做病理切片,观察肝细胞脂变情况。结果:与正常组比较,模型组超声图像近区、远区灰度显著增加,肝脏TG、TC明显升高。组织病理学分析可见模型大鼠肝脏典型脂肪样变。结论:超声量化技术可作为一种无创性量化方法用于判断大鼠肝脏脂肪变化。Objective： To explore the quantitive assessment of fatty liver of rats by measuring ultrasonic gray scale. Methods： A mixture composed of alcohol, lard and cholesterol was supplied to rats for 12 weeks in order to establish a rat model of hepatic steatosis. To measure ultrasonic gray scale of liver and hepatic pathological feature liver, and the changes of the contents of triglyeeride （TG） and cholesterol （TC） in liver tissue were observed. Results： Gray scale from near or far areas of ultrasonic image in model rats was higher than that of normal rats. And the rats of fatty liver diseases had a significantly higher level of TG. and TC in liver tissue than those of normal rats. The typical fatty liver pathologic changes were observed in the model rats. Conclusion： Hepatic ultrasonic gray scale can reflect the grades of hepatic steatosis, which is served as reliable noninvasive tools for the quantitative diagnosis of fatty liver disease.

关 键 词：超声检查 量化 灰阶强度 脂肪肝 

分 类 号：R965.2[医药卫生—药理学]