牛蒡子中牛蒡子苷元的分离及结构表征  

Separation and structural characterization of arctigenin in Arctii Fructus

作　　者：周渭渭[1,2] 刘振国[3] 单淇[1] 张新鑫[1] 周鸿[3] 辛宁[2] 侯文彬[1] 

机构地区：[1]天津药物研究院,天津市中药质量控制技术工程实验室,天津300193 [2]广西中医学院,广西南宁530001 [3]天津药物研究院药业有限责任公司,天津300193

出　　处：《药物评价研究》2010年第4期279-283,共5页Drug Evaluation Research

基　　金："十一五"国家科技支撑计划资助项目(2007BAI41B06)

摘　　要：目的:从牛蒡子中分离纯化出有效成分,并对其分子结构进行鉴定与表征。方法:采用醇提酸解法提取,分离出粗品,经过对粗品进行脱脂、结晶处理,得到无色棱状晶体。采用紫外光谱(UV)、红外光谱(FTIR)、质谱(MS)、氢谱(H-NMR)、碳谱(C-NMR)及单晶衍射对该有效成分进行组成和分子结构的鉴定与表征。结果:从牛蒡子粗粉中经提取分离,得到结晶,提取率为4.46%,显色反应及波谱分析均显示为木脂素类化合物。结论:该有效成分鉴定为牛蒡子苷元,即(3R,4R)-4-[(3,4-二甲氧基苯基)甲基]二氢-3-[(4-羟基-3-甲氧基苯基)甲基]-2(3H)-呋喃酮,分子式为C21H24O6。Objective： To isolate and purify active component from Arctii Fructus and identify and characterize. Methods： The crude product was separated from Arctii Fructus by alcohol extraction and acid hydrolysis. Using recrystallization its structure was method, colorless prismatic crystal was obtained. The composition and molecular structure were characterized by UV, FTIR, MS, ^1H-NMR, ^13C-NMR, and X-ray analyses. Results： The extracting rate of the crystal gained from the powder of Arctii Fructus was 4.46%. Both color reactions and spectral analyses indicated that the compound was lignan, Conclusion： The active component is characterized as （3R, 4R）-4-[（3,4-dimethoxyphenyl） methyl] dihydro-3-[（4-hydroxy-3-methoxy phenyl） methyl]-2（3H）-furanone （arctigenin, ARC-G） and the molecular formula is C21H24O6.

关 键 词：牛蒡子 牛蒡子苷元 分离 结构表征 

分 类 号：R284.1[医药卫生—中药学]