用硼酸衍生物检测产质粒AmpC酶  

Using a boronic acid derivative for identification of AmpC β-Iactamase-producing isolates

作　　者：马卫红[1] 

机构地区：[1]河南省濮阳市油田总医院检验科,457001

出　　处：《国际检验医学杂志》2010年第8期792-793,796,共3页International Journal of Laboratory Medicine

摘　　要：目的调查用3-氨基苯硼酸检测大肠埃希菌、肺炎克雷伯菌产质粒AmpC酶的实用性。方法菌株鉴定用API20E系统。用纸片增效试验、双纸片协同试验、微量稀释试验检测产质粒AmpC酶。结果用3-氨基苯硼酸与纸片增效试验、双纸片协同试验、微量稀释试验结合检测大肠埃希菌、肺炎克雷伯菌的质粒AmpC酶,产酶率分别为23.8%(96/403)、22.8%(92/403)、24.3%(98/403);1 6.9%(38/225)、16.9%(38/225)、18.22%(41/225)。5.3%(33/628)产质粒AmpC酶对头孢西丁敏感。1.8%(11/628)的大肠埃希菌和肺炎克雷伯菌同时产质粒AmpC酶和ESBLs。结论采用3种方法与3氨基苯硼酸结合检测产质粒AmpC酶,操作简单,灵敏度高,特异性强,可作为临床微生物实验室的常规工作。纸片增效试验、双纸片协同试验更适合于基层医院。用头孢西丁筛选产质粒AmpC酶的方法存在一定缺陷。Objective To investigate the practicality using 3-aminophenylboronic acid（APB） for identification of plasmid-me- diated AmpC β-iactamase producers among Eschericbia coli,Klebsiella pneumoniae isolates. Methods Isolates were identified with the API 20E systems. AmpC β-lactamase production was detected by the disk potentiation test, the double-disk synergy test,the microdilution test with 3 aminophenylboronic acid. Results The disk potentiation test, the double-disk synergy test,the microdilu tion test with APB,the rate of producing AmpC β--1actamase of Escherichia coli, Klebsiella pneumoniae isolates were 23.8% （96/ 403）,22.8%（92/403）,24. 3% （98/403） ; 16. 9% （38/225）, 16. 9% （38/225）, 18. 22%（41/225）, respectively 5. 3% （33/628） of AmpC producers were found to be susceptible to cefoxitin. 1.8% （11/628） clinical isolates coproduce produced AmpC β-iactamase and extended-spectrum β-lactamase. Conclusion All three tests, the disk potentiation test,the double-disk synergy test,and the microdilution test with APB, were very simple, highly sensitive,and specific for the identification of isolates producing AmpC β-iactamases. They are fully applicable for routine use in clinical microhiology laboratories. The disk potentiation test and double-disk synergy test are more suitable for identification of plasmid-mediated AmpC β-lactamase producers among Escherichia coli, Klebsiella pneumoniae isolates for the matrical hospital. Screening methods that use cefoxitin to detect plasmid-mediated AmpC β-lctamase-producing isolates are not perfect.

关 键 词：硼酸类 头孢菌素酶 埃希氏菌属 克雷伯菌 肺炎 

分 类 号：R446.5[医药卫生—诊断学]