丙戊酸钠协同阿霉素抑制MUTZ-1细胞株增效作用的研究  

Inhibition of Adriamycin Combined with Sodium Valproate on MUTZ-1 Cell Line

作　　者：俞晨[1] 陈宝安[2] 

机构地区：[1]东南大学附属南京市第二医院普内科,江苏南京210003 [2]东南大学附属中大医院血液科,江苏南京210009

出　　处：《现代生物医学进展》2010年第16期3033-3036,共4页Progress in Modern Biomedicine

基　　金：国家自然基金资助项目(30070318)

摘　　要：目的:探讨丙戊酸钠协同阿霉素对MUTZ-1细胞株生长的抑制作用及其作用机制。方法:采用四甲基偶氮唑蓝(MTT)比色法观察药物对细胞生长的影响;发光比色法检测Caspase-3的活性。结果:小剂量丙戊酸钠可协同阿霉素抑制MUTZ-1细胞株生长,细胞生长抑制率在两药联合作用后比单独使用阿霉素明显增加,有剂量依赖性,与对照组相比有显著统计学意义。单药使用阿霉素时细胞的caspase-3活性上升,两药联合后caspase-3活性上升更明显。结论:小剂量的丙戊酸钠能增敏阿霉素诱导MUTZ-1细胞株发生凋亡,作用机制可能是通过上调caspase-3的活性来完成的。Objective： To investigate the tumor suppression efficacy of histone deacetylase inhibitor,valproate sodium,in combination with Adriamycin in the treatment of myelodysplastic syndrome cell line MUTZ-1 and its mechanism. Methods： Logarithmic growth phase MUTZ-1 cell line was treated on different density of valproic acid,Adriamycin,alone or combination;after alone and combination. Caspase-3 activity was detected. Results： 0.25mmool/l valproate sodium did not show apoptosis effect, but can synergize Adriamycin to promote Apoptosis. it was concentration -dependent. All the data above were significantly different from that in control （ p〈 0.05）. Aider treated with SVPA and ADM for 72h, the level ofcaspase-3 in MUTZ-1 was enhanced （P〈0.05）. Conclusions： valproate sodium may enhance the efficacy of ADM in MUTZ-1 cell line. the mechanism of action is possibly through the activity ofcaspase-3.

关 键 词：丙戊酸钠 骨髓增生异常综合症 MUTZ-1细胞株 细胞凋亡 

分 类 号：R551.3[医药卫生—血液循环系统疾病] R730.5[医药卫生—内科学]