奶牛胎衣不下与胎儿胎盘中iNOS、eNOS及MMP-2表达的相关性  

Relationship of Retained Fetal Membranes of Cows to Expressions of iNOS, eNOS and MMP-2 in Placenta

作　　者：付世新[1] 张丽[1] 齐长学[1] 罗春海[1] 李鹏[1] 夏成[1] 

机构地区：[1]黑龙江八一农垦大学动物科技学院,黑龙江大庆163319

出　　处：《中国生物制品学杂志》2010年第8期825-828,838,共5页Chinese Journal of Biologicals

基　　金：黑龙江省青年科学技术专项资金项目(QC06C001)

摘　　要：目的分析奶牛胎衣不下(Retained fetal membranes,RFM)与胎儿胎盘中诱导型一氧化氮合酶(Induction nitric oxid esynthase,iNOS)、内皮型一氧化氮合酶(Endothelial nitric oxide synthase,eNOS)和基质金属蛋白酶-2(Matrix metallopeptidase-2,MMP-2)表达的相关性,探讨RFM发生的分子机制。方法 RT-PCR扩增、克隆产后胎衣正常脱落(No retention of fetal membranes,NRFM)和RFM奶牛胎儿胎盘中iNOS、eNOS和MMP-2基因,以牛β-actin基因为内参,采用荧光定量PCR法检测RFM和NRFM奶牛胎儿胎盘组织中iNOS、eNOS和MMP-2基因表达的差异。结果克隆的iNOS、eNOS和MMP-2基因片段与GenBank中登录的核苷酸序列同源性均达100%。RFM组iNOS基因mRNA的转录水平明显高于NRFM组(P<0.05),eNOS和MMP-2基因mRNA的转录水平则明显低于NRFM组(P<0.05)。结论 iNOS、eNOS和MMP-2基因mRNA的转录水平与RFM的发生密切相关。Objective To analyze the retained retention of fetal membrane（RFM） of cows to the expressions of induction nitric oxide synthase （iNOS）, endothelial nitric oxide synthase （eNOS）and matrix metallopeptidase-2 （MMP-2）in placenta as well as the molecular mechanism of onset of RFM. Methods The iNOS, eNOS and MMP-2 genes were amplified by RT-PCR from the placentas of cows with no RFM （NRFM）and with RFM separately and cloned, using bovine β-actin as an internal reference, and the expression levels were determined by fluorescent quantitative PCR. Results The iNOS, eNOS and MMP-2 genes were cloned, of which the homologies of nucleotide sequences were 100% to those reported in GenBank. The transcription level of iNOS mRNA in the placenta of cows in RFM group was significantly higher, while those of eNOS and MMP-2 mRNAs were significantly lower than those in NRFM group（P 0. 05）. Conclusion The transcription levels of iNOS, eNOS and MMP-2 mRNAs were closely related to the onset of RFM.

关 键 词：胎衣不下 一氧化氮合酶 基质金属蛋白酶2 荧光定量PCR 

分 类 号：S858.237.2[农业科学—临床兽医学]