分光光度法测定炭疽疫苗的浓度  

Determination of Concentration of Anthrax Vaccine by Spectrophotometry

作　　者：魏东[1] 王秉翔[2] 李恪梅[1] 裴明玉[2] 李晓莉[2] 王国治[1] 

机构地区：[1]中国药品生物制品检定所细菌一室,北京100050 [2]兰州生物制品研究所,兰州730046

出　　处：《中国生物制品学杂志》2010年第8期894-895,905,共3页Chinese Journal of Biologicals

摘　　要：目的应用分光光度法测定炭疽疫苗的浓度。方法确定分光光度法测定炭疽疫苗的测定波长及线性范围,并对样品稳定性及方法的重复性进行分析。结果确定600nm作为检测波长;炭疽疫苗浓度在(0.25～1.25)×108个/ml之间时,A600值与菌液浓度之间具有良好的相关性(r=0.9991,自由度=3,P<0.01);样品经甲醛处理后稳定性增强;该方法具有良好的重复性。结论分光光度法可用于炭疽疫苗浓度的测定,可替代比浊法或作为炭疽疫苗浓度测定的确证方法。Objective To determine the concentration of anthrax vaccine by spectrophotometry. Methods The wavelength and linear range for spectrophotometry of anthrax vaccine were determined, based on which the stability of test samples and repro - ducibility of the method were analyzed. Results The wavelength for spectrophotometry of anthrax vaccine was determined as 600 nm. The A600 value showed a good relationship（r = 0. 999 1, df = 3, P 0. 01）to anthrax vaccine concentration at a range of（0. 25 ～ 1. 25）× 108 bacteria / ml. The stability of test samples increased after treatment with formaldehyde. The method showed high reproducibility. Conclusion Spectrophotometry may be used for determination of anthrax vaccine concentration instead of turbidimetry or as a method for confirmation.

关 键 词：炭疽 疫苗 分光光度法 

分 类 号：O657.32[理学—分析化学] R378.72[理学—化学]