多西他赛对肺癌A549细胞中eIF3a表达的影响(英文)  

Effect of docetaxel on expression of eIF3a in human lung cancer A549 cell line

作　　者：龚志诚[1,2] 徐潇静[1] 侯瑞英[1] 郭玥[3] 盛飞凤[1] 欧阳冬生[1] 周宏灏[1] 刘昭前[1] 

机构地区：[1]中南大学临床药理研究所,遗传药理学湖南省重点实验室,长沙410078 [2]中南大学湘雅医院药剂科,长沙410008 [3]中国药科大学药学院,南京211198

出　　处：《中南大学学报（医学版）》2010年第8期771-776,共6页Journal of Central South University ：Medical Science

基　　金：supported by the Program for Changjiang Scholars and Innovative Research Team in University (IRT0946);Nature National Foundation of China (30873089);the Nature Science Foundation of Hunan Province, P. R. China(08JJ3058)

摘　　要：目的:探讨人类肺癌细胞株中,不同剂量的多西他赛对真核翻译起始因子3a(eukaryotic in-itiation factor 3 subunit a,eIF3a)和α-微管蛋白(α-tubulin)表达的影响及其时效、量效关系。方法:人类肺腺癌细胞株A549用不同浓度的多西他赛处理不同的时间后,Real-time PCR法检测α-微管蛋白和eIF3a的mRNA表达水平,Western印迹法检测α-微管蛋白和eIF3a的蛋白表达水平。结果:多西他赛不能影响α-微管蛋白mRNA和蛋白水平的表达,且eIF3a与α-微管蛋白的表达水平之间没有明显的相关性。高浓度(30μg/L)多西他赛处理细胞后,eIF3a的mRNA表达水平随着时间的延长有上升的趋势。结论:多西他赛在高剂量时具有增加eIF3amRNA表达的趋势,eIF3a不参与α-微管蛋白表达的调节。Objective To explore the dose-dependent and time-dependent effect of docetaxel on the expression of mammalian eukaryotic initiation factor 3 subunit A（eIF3a） in lung cancer cell line.Methods The human lung cancer cell line A549 was treated with gradient concentrations of docetaxel for different time.Real-time PCR and Western blot were used to detect mRNA and protein expression levels of eIF3a and α-tubulin,respectively.Results Docetaxel did not affect α-tubulin expression at either mRNA level or protein level.When A549 cells were treated with high concentration of docetaxel（30 μg/L）,the expression level of eIF3a mRNA tended to increase in a time-dependent manner.Protein expression level of α-tubulin was not associated with eIF3a expression significantly in cells treated by docetaxel.Conclusion Docetaxel could slightly increase the expression of eIF3a mRNA,and eIF3a does not regulate the expression of α-tubulin in A549 cells treated by docetaxel.

关 键 词：多西他赛 eIF3a Α-微管蛋白 肺癌 

分 类 号：R817.4[医药卫生—影像医学与核医学]