精液留取后不同时间分析对精子运动参数的影响  

Effects of semen analysis on human sperm movement parameters at different times after semen collection

作　　者：邵永[1] 戈一峰[1] 赵晓峰[2] 姚兵[1] 陆金春[3] 崔英霞[1] 商学军[1] 夏欣一[1] 黄宇烽[1] 

机构地区：[1]南京军区南京总医院解放军临床检验医学研究所,江苏南京210002 [2]内蒙古自治区医院检验科,内蒙古呼和浩特010017 [3]武警江苏总队医院南京分院检验科,江苏南京210028

出　　处：《中华男科学杂志》2010年第7期631-634,共4页National Journal of Andrology

摘　　要：目的:探讨精液留取后不同时间进行计算机辅助精液分析系统(CASA)分析对精子运动参数的影响。方法:选择92例精液标本,精子密度均大于20×106/ml,液化时间均小于20min。精液留取后置37℃孵箱,在20、30、60、90min时,采用CASA进行精液参数分析。结果:30、60、90min时a级精子百分率、b级精子百分率分别显著低于20min时的a级精子百分率、b级精子百分率(P<0.05)。60min、90min时c级精子百分率分别显著低于20min、30min时的c级精子百分率(P<0.05)。c级精子百分率在20min和30min时无明显差异(P>0.05)。30、60、90min时(a+b)级精子百分率、(a+b+c)级精子百分率则均显著低于20min时(a+b)级精子百分率、(a+b+c)级精子百分率(P<0.05)。鞭打频率(BCF)在30min时则显著高于20min(P<0.05)。90min时侧摆幅度(ALH)与30min时相比显著降低(P<0.05)。90min时摆动性(WOB)与20、30min时比显著增高(P<0.05)。90min时曲线速度(VCL)显著低于20、30min时的曲线速度(P<0.05)。30、60、90min时的前向性(STR)与20min相比显著降低(P<0.05)。9min时的平均路径速度(VAP)、直线速度(VSL)分别均显著低于20min时的平均路径速度、直线速度(P<0.05)。精子密度、平均移动角度(MAD)、直线性(LIN)在4个不同时间点分析时均无显著性差异(P>0.05)。结论:精液常规分析中精液留取后至分析的时间需要标准化,对正常液化标本,精子留取后30～60min精子运动参数分析相对恒定。Objective： To investigate the effects of the computer-assisted semen analysis （CASA） on human sperm movement parameters at different times after semen collection. Methods： Ninety-two semen samples with sperm density ≥ 20 × 10^6/ml and sperm liquefaction time 〈 20 min were placed in a incubation box at the temperature of 37℃. Then the seminal parameters were analyzed with the computer-assisted semen analysis （CASA） system at 20, 30, 60 and 90 min after semen collection. Results ： The percentages of grade a and b sperm were significantly lower at 30, 60 and 90 min than at 20 min （ P 〈 0.05 ）, so were the percentages of grade c sperm at 60 and 90 min than at 20 and 30 min （ P 〈 0.05 ）, but there were no significant differences in the percentage of grade c sperm between the 20-min and 30-min groups （ P 〉 0.05 ）. The percentages of grade a ＋ b and a ＋ b ＋ c sperm were also significantly lower at 30, 60 and 90 rain than at 20 min （P 〈 0.05）. The beat cross frequency （BCF） was significantly higher at 30 rain than at 20 rain （P 〈 0.05 ）, while the lateral head amplitude （ALH） significantly lower at 90 min than at 30 rain （P 〈 0.05）. The sperm wobbliness （WOB） was significantly higher while the curvilinear velocity （VCL） significantly lower at 90 min than at 20 and 30 rain （P 〈 0.05 ）. Straightness （STR） at 30, 60 and 90 min, and average path velocity （VAP） and straight line velocity （VSL） at 90 rain were significantly lower than at 20 rain （P 〈 0.05）. There were no significant differences in sperm density, average motion degree （MAD） and linearity （LIN） among the four groups （ P 〉 0. 05 ）. Conclusion ： The interval between semen collection and sperm routine analysis needs to be standardized. The results of this study suggest that sperm movement parameters of normal liquefied semen samples are relatively constant at 30 -60 min after semen collection. Natl J Androl, 2010, 16 （7） ： 631 -634

关 键 词：精子 运动参数 计算机辅助精液分析 标准化 

分 类 号：R698.2[医药卫生—泌尿科学]