正丁醇和高光强下转玉米pepc基因水稻叶片超微结构的变化  

Change of Micro-structure in Transgenic Rice of pepc Gene from Maize with High Photo-efficiency under the Strong Light with 1-Butanol Treatment

作　　者：李霞[1] 王超[1] 任承刚[1] 丛伟[1] 金磊[1] 郭士伟[1] 

机构地区：[1]江苏省农业科学院粮食作物研究所江苏省优质水稻工程技术研究中心

出　　处：《西北植物学报》2010年第8期1614-1621,共8页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金项目(30871459);转基因生物新品种培育科技重大专项(2008ZX08001-004);江苏省自主创新项目[cx(09)109]资助

摘　　要：以高光效高产的转玉米pepc基因水稻（PC）和野生型水稻（WT）为研究材料,在水稻苗期进行高光强处理（120 min,1 000μmol·m^-2·s^-1、高光强正丁醇复合处理（120 min,1 000μmol·m^-2·s^-10.04%正丁醇）和正常光强处理（200μmol·m^-2·s^-1CK）,运用透射电镜观察各处理材料叶肉细胞、维管束鞘、叶绿体、叶绿体片层以及线粒体等结构变化特点,并考察它们叶片的净光合速率（Pn）、磷酸烯醇式丙酮酸羧化酶（PEPC）活性以及收获后的产量构成因子等.结果发现：PC植株具有较高Pn和PEPC活性,与其较高的有效穗数、穗长、千粒重和籽粒产量相对应;与野生型（WT）相比,高光强处理的PC植株叶肉细胞完整,维管束鞘细胞排列整齐,类囊体片层厚,排列有序,堆叠整齐,并且线粒体有序地在叶绿体周围聚集;但经正丁醇和高光强复合处理的PC叶绿体类囊体片层则降解,淀粉粒累积,表现出类似于高光强下WT植株被损坏的超微结构特征.研究表明,转玉米pepc基因水稻叶片的叶肉细胞、叶绿体和线粒体等具有高光效的超微结构特征;PEPC可能通过磷脂酶D（PLD）途径产生的磷脂酸（PA）参与PC在高光强下对类囊体片层稳定性的调节.pepc transgenic rice（PC） with high photosynthesis and yield and its wild type（WT） at seedling stage were treated under 1 000 μmol·m^-2·s^-1ght intensity for 120 min and treated with 0.04% 1-butanol under 1 000 μmol·m^-2·s^-1ght intensity for 120 min,respectively,and those under 200 μmol·m^-2·s^-1ght as the control.The ultrastructure of mesophyll cells,bundle sheath,mitochondria and thylakoids of the second blades in PC and WT were observed with a transmission electron microscope.And the net photosynthetic rate（Pn）,PEPC activity and their yield components were measured after harvesting.The results showed that the higher Pn was similar with the higher yield in PC.Furthermore the ultrastructure of the plants exhibited that,compared with the control of PC,the mesophyll cells of PC after the treatment of 1 000 μmol·m^-2·s^-1ght intensity for 120 min were completed,the cells of bundle sheath were ordered,the ordered lamella in chloroplast were thicker and arranged denser and the mitchondia among the chloroplast increased after strong light treatment.But,after the treatment of 1-butanol under 1 000 μmol·m^-2·s^-1ght intensity for 120 min,the lamella of thylakoids in PC were looser and the structure of them were confusion even disorganization like those performance of WT just under strong light.It indicated that PC under strong light can keep a steady photosynthetic structure to avoid the damage from strong light,which might be related with PLD/PA signaling pathway.This paper indicated the biological ultrastructure basis of transgenic rice plants expressing maize pepc gene with high photosynthesis efficiency.

关 键 词：转玉米pepc基因水稻 叶肉细胞 类囊体片层 线粒体 正丁醇 高光强 

分 类 号：Q248[生物学—细胞生物学]