转反义trxs基因小麦籽粒后熟过程中水解酶活性的变化  

Change of Hydrolase Activities in Transgenic Anti-sense Thioredoxin s Gene Wheat in the Process of After-ripening

作　　者：任江萍[1] 尹钧[1] 周苏玫[1] 李永春[1] 牛洪斌[1] 

机构地区：[1]河南农业大学/国家小麦工程技术研究中心

出　　处：《西北植物学报》2010年第8期1634-1638,共5页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金项目(30771332)

摘　　要：以小麦品种生抗1号（对照）及其转反义trxs基因纯合株系为材料,测定了它们在种子后熟过程中硫氧还蛋白h（Trxh）、淀粉酶和蛋白酶活性以及呼吸速率等生理指标,以探讨转反义trxs基因小麦籽粒在后熟过程中的生理生化变化规律,为提高小麦耐储性探索有效途径.结果显示,在小麦后熟过程中,转基因小麦籽粒的Trxh活性、淀粉酶活性、蛋白水解酶活性和种子呼吸速率均比对照明显下降;后熟0～30 d,转基因小麦种子的Trxh活性、α-淀粉酶、β-淀粉酶、脱支淀粉酶、蛋白酶活性和种子呼吸速率平均比对照分别降低16.07%、31.64%、6.44%、21.63%、27.67%和52.90%.研究表明,反义trxs基因导入干扰或部分抑制了小麦内源同源基因的表达,致使小麦种子Trxh活性、呼吸速率和水解酶活性降低,从而延缓了籽粒储藏物质的转化速率,提高了小麦种子的耐贮藏性.In order to exploring a effective way for improving wheat seed storage,we detected the seed respiration speed and the activities of thioredoxin h,α-amylase,β-amylase,debranching enzyme and protease in transgenic line and non-transgenic cultivar ＇Shengkang No.1＇ by enzyme activity assay during seed after-ripening stage.The results indicated that the activities of thioredoxin h,α-amylase,β-amylase,debranching enzyme and protease were significantly lower and the seed respiration velocities decreased distinctly in transgenic line.During after-ripening 0～30 d,the activities of thioredoxin h,α-amylase,β-amylase,debranching enzyme,protease and seed respiration velocities were 16.07%,31.64%,6.44%,21.63%,27.67% and 52.90% lower than that of non-transgenic seeds,respectively.The above result demonstrated that foreign trxs gene interfered the expression of the endogenous gene,decreased Trxh activity,seed respiration velocities and hydrolyses activities and retarded converting velocities of reserve materials.Therefore,the seed storability was improved.

关 键 词：小麦 反义TRXS基因 转基因 生理特性 

分 类 号：Q945.65[生物学—植物学]