微卫星技术在大耳白黑眼兔近交系培育中的监测分析  

Monitoring Inbreeding of WHBE Rabbits Using Microsatellites

作　　者：陈民利[1] 朱亮[1] 蔡月琴[1] 余佳[1] 徐孝平[1] 屠珏[1] 肖慧[1] 潘永明[1] 

机构地区：[1]浙江中医药大学动物实验研究中心/比较医学研究中心,浙江杭州310053

出　　处：《Zoological Research》2010年第4期401-407,共7页动物学研究（英文）

基　　金：国家自然科学基金项目(30770322);浙江省卫生高层次创新人才培养工程项目

摘　　要：利用微卫星技术监测大耳白黑眼兔(white hair black eyes rabbits,WHBE兔)近交培育中第五代(F5)、第六代(F6)和第七代(F7)的遗传多样性。选取21个微卫星座位,筛选出扩增产物稳定并且具有多态性的11对微卫星引物用于本研究。结果表明,F5代WHBE兔在每个座位上的等位基因数(Na)为3～9个不等,11个座位的平均有效等位基因数(Ne)为1.81个,平均观察杂合度(Ho)和平均多态信息含量(PIC)分别为0.381和0.524,累积个体识别率(CDP)达到100%,累积非父排除概率(CPE)在双亲信息都是未知情况下的为0.926,而在得知任一亲本信息的情况下,CPE值为0.993。F6代WHBE兔在每个座位上的Na为3～8个不等,11个座位的平均Ne为1.68个,平均Ho和PIC值分别为0.356和0.548,CDP达到100%,CPE在双亲信息都是未知情况下的为0.931,而在得知任一亲本信息的情况下,CPE值为0.994。F7代WHBE兔在每个座位上的Na为2～6个不等,11个座位的Ne为1.51个,平均Ho和PIC值分别为0.287和0.498,CDP达到100%,CPE在双亲信息都是未知情况下的为0.891,而在得知任一亲本信息的情况下,CPE值为0.986。在近交系培育过程中,从F5代到F7代,WHBE兔的平均Ne和平均Ho都呈下降趋势,提示随着近交代数的增加,WHBE兔的基因纯合度越来越高。Microsatellite polymorphisms were analyzed in F5,F6 and F7 WHBE rabbits（Oryctolagus cuniculus） to monitor inbreeding.Out of 21 microsatellite loci,11 were successfully amplified and showed polymorphic.For the F5 WHBE rabbits,the number of alleles per locus（Na） ranged from 3 to 9 and the mean effective number of alleles（Ne） was 1.81.The mean value of observed heterozygosity（Ho） and polymorphism information content（PIC） of the 11 polymorphic loci were 0.381 and 0.524,respectively.The power of cumulative discrimination（CDP） was 1.0.The value of cumulative exclusion probability of the 11 polymorphic loci in the absence（CPE-1） or in the presence of genetic information on the first parent（CPE-2） was 0.926 and 0.993,respectively.For the F6 WHBE rabbits,Na per locus ranged from 3 to 8 and the mean value of Ne was 1.68.The mean value of Ho,PIC,CDP,CPE-1,CPE-2 of the 11 polymorphic loci were 0.356,0.548,1.0,0.931,0.994 respectively.For the F7 WHBE rabbits,Na per locus ranged from 2 to 6 and the mean value of Ne was 1.51.The mean value of Ho,PIC,CDP,CPE-1,CPE-2 of the 11 polymorphic loci were 0.287,0.498,1.0,0.891,0.986 respectively.The average number of effective alleles and the average observed heterozygosity were decreasing continuously in F5,F6 and F7,suggesting that the purity of WHBE rabbits was increasing continuously.

关 键 词：大耳白黑眼兔 近交系培育 微卫星 遗传多态性 

分 类 号：S829.1[农业科学—畜牧学]