珠美海棠MzWRKY基因家族盐胁迫应答模式研究  

Expression Analysis of WRKY Family in Response to Salt Stress in Malus zumi Mats

作　　者：蒋阿维[1] 张素维[1] 孙杨吾[1] 褚欣然[1] 赵宇[1] 许雪峰 孔瑾[1] 

机构地区：[1]中国农业大学农学与生物技术学院,北京市果树逆境生理与分子生物学实验室,北京100193

出　　处：《园艺学报》2010年第8期1213-1219,共7页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(30600416);北京市自然科学基金项目(5072027);中央高校基本科研业务费专项(2009-2-06)

摘　　要：对苹果属植物现有的58个WRKY的EST序列进行分析,得到了37条uniESTs。珠美海棠(Malus zumi Mats)是耐盐的优良苹果砧木,能在土壤全盐含量为0.6%的盐碱地上存活。根据已知的37条uniESTs序列,通过半定量RT-PCR研究了珠美海棠中MzWRKY基因家族的盐应答模式。28个MzWRKY基因能检测到RT-PCR的产物,其中21个基因受盐诱导,1个受盐抑制。根据MzWRKY基因盐应答模式可将这些基因分为两组,基因表达模式的差异说明MzWRKY在珠美海棠的盐应答中角色的多样性。Sequence assembly from 58 Malus WRKY ESTs resulted in 37 uniESTs. Malus zumi Mats is a salt-tolerant rootstock of apple, which could survive in 0.6% total salt content of land. Primers were designed in M. zumi Mats according to the 37 uniESTs, semi-quantitative RT-PCR was applied to reveal the expression patterns of MzWRKY gene family in response to salt stress. RT-PCR products of 28 MzWRKY genes were detectable, 21 MzWRKYs were up-regulated and 1 M_zWRKY was down regulated under salt stress. According to the peak hour, the MzWRKYs were classified into 2 groups. The different expression patterns of MzWRKY genes suggested that WRKY proteins play various roles in response to salt stress in M. zumi Mats.

关 键 词：珠美海棠 WRKY转录因子 盐胁迫 半定量RT-PCR 

分 类 号：S661[农业科学—果树学]