外源多胺在莴苣幼苗侧根发育中的作用及其与一氧化氮的关系  

The Induced Effects of Exogenous Polyamines on the Development of Lettuce Seedling Lateral Roots and Their Relationship with Nitric Oxide

作　　者：孙娜[1] 王立伟[1] 张凤芝[1] 苏国兴[1] 

机构地区：[1]苏州大学基础医学与生物科学学院,江苏苏州215123

出　　处：《园艺学报》2010年第8期1273-1278,共6页Acta Horticulturae Sinica

基　　金：苏州大学博士点基金项目(13120740)

摘　　要：以莴苣(Lactuca sativa L.)‘挂丝红’种子为材料,研究多胺(PAs)和一氧化氮(NO)在莴苣幼苗侧根发生过程中的作用以及它们两者的相互关系。结果表明,低浓度的外源多胺在提高莴苣幼苗体内多胺的同时,可显著促进幼苗侧根的发育,且以0.05mmol·L-1处理最佳。多胺生物合成抑制剂环己胺(CHA)和甲基-(乙二醛)-双-鸟嘌呤腙(MGBG)可明显抑制幼苗侧根的发育。外源NO供体硝普钠(SNP)处理显著促进莴苣幼苗侧根的发育,呈现与多胺相似的性质。在外源多胺和SNP处理的同时,添加NO清除剂2,4-羧基苯-4,4,5,5-四甲基咪唑-1-氧-3-氧化物(cPTIO)可显著降低SNP和外源多胺对侧根发育的促进作用。用NO专性荧光探针1,2-二氨基蒽醌(DAQ)检测表明,在多胺和SNP处理后侧根原基突起部位均有较强的NO荧光产生,而cPTIO可降低多胺和SNP处理引起的荧光产生,并伴随对侧根发育的抑制。可见在多胺促进莴苣幼苗侧根发育过程中,NO可能参与多胺的信号转导。Using lettuce seeds （Lactuca sativa L. ＇Guasihong＇ ） as a material, the role of exogenous polyamines （PAs） and nitric oxide （NO） in the process of lateral root development in lettuce seedlings and their relationship were investigated. As a result, treatments with exogenous PAs at low concentrations were able to increase the levels of endogenous PAs and to promote the formation and development of lateral roots, and their optimal concentrations were 0.05 mmol· L^-1. Consistently inhibitors of polyamines biosynthesis cyclohexylamine （CHA） and methyl-glyoxal-bis-guanyhyldrazone （MGBG） significantly reduced the development of lettuce lateral roots. Like PAs, exogenous NO donor sodium nitroprusside （SNP）also promoted the development of lettuce laterals. When treated with SNP and PAs, the application of NO scavenger 2- （4-carboxyphenyl） -4, 4, 5, 5-tetramethylimidazoline- 1-oxyl-3-oxide （cPTIO） could markedly prevented the SNP- or PAs- promoted effects on lateral roots. Fluorescent detection using the 1,2-diaminonanthraquinone（DAQ）, a NO-sensitive fluorescent probe, showed that lateral root primordia treated with PAs or SNP emitted strong NO fluorescence, which could be eliminated by cPTIO, concomitantly with its inhibitory effect on lateral root emergence promoted by PAs or SNP. All together, these results suggest that NO is probably involved in PAs signaling pathway during the process of PAs induced development of lateral roots in lettuce seedlings.

关 键 词：莴苣 多胺 一氧化氮 侧根发育 

分 类 号：S636.2[农业科学—蔬菜学]