漆黄素对巨噬细胞和T淋巴细胞的免疫调节作用  

Immune regulation of fisetin on macrophages and T lymphocytes

作　　者：陈真[1] 曾耀英[1] 叶燕霞[1] 

机构地区：[1]暨南大学生命科学技术学院组织移植与免疫实验中心,广州510632

出　　处：《免疫学杂志》2010年第8期653-656,共4页Immunological Journal

基　　金：国家重点基础研究发展计划资助项目(2006CB504201;2004CB720100);广东省自然科学基金资助项目(5300419);广州市科技局科技攻关重点项目(2006Z-E0091)

摘　　要：目的探讨漆黄素(Fisetin,FIS)对小鼠巨噬细胞吞噬功能、NO的释放及对T淋巴细胞体外活化、增殖的影响。方法无菌制备小鼠巨噬细胞悬液及淋巴细胞悬液;荧光微球结合流式细胞术(FCM)分析FIS对巨噬细胞吞噬作用的影响;Griess试剂盒检测巨噬细胞NO的释放;双色荧光抗体染色结合FCM,检测CD3+T细胞CD69的表达水平;CFSE标记技术检测T细胞增殖的情况。结果 2.5μmol/L,5μmol/L,10μmol/LFIS均能明显抑制巨噬细胞的吞噬微球的能力;FIS能抑制LPS和IFN-γ刺激的巨噬细胞的NO的产生(P<0.05);FIS对ConA刺激的T细胞表达CD69有抑制作用,并能有效抑制ConA诱导的T细胞增殖(P<0.01),且均呈剂量依赖性。结论 FIS能显著抑制小鼠腹腔巨噬细胞的吞噬能力和分泌NO的能力,并能够抑制T细胞的活化和增殖,有望发展成为一种新的免疫抑制药物。This study aims to investigate the effects of fisetin （FIS） on the mouse macrophages and T lymphocytes in vitro.Firstly,macrophages and lymphocytes of mice were prepared,and then stimulated with Fisetin.Fluorescence microbeads plus flow cytometry were used to analyze phagocytosis of macrophages of mouse;NO secretion of macrophages was detected by Griess reagent system;the expression of CD69 on CD3＋T lymphocytes was measured by flow cytometry combined with two colored monoclonal antibodies;the effect of FIS on the proliferation of T lymphocytes was determined by CFSE staining technology.We found that NO production and phagocytosis rate of macrophages were inhibited by Fisetin （P 0.05）;the expression of CD69 was significantly inhibited by Fisetin （P 0.01）.Our results also showed that FIS （2.5 μmol/L,5 μmol/L and 10 μmol/L） significantly inhibited the proliferation of T lymphocytes（P 0.01）.These data suggest that FIS can significantly inhibit the NO production and phagocytosis of macrophages,as well as can inhibit the proliferation and activation of T lymphocytes,thus promising to be developed as an immusuppressive drug.

关 键 词：漆黄素 吞噬 一氧化氮 增殖 活化 

分 类 号：R285[医药卫生—中药学]