细菌磁小体的修饰及其在病原物检测中的应用  

Modification of Bacterial Magnetosomes and Application of Magnetosome-Antibody Complex in Pathogen Detection

作　　者：李爱华[1] 唐涛[1] 张惠媛[2] 汪琦[2] 田杰生[1] 李颖[1] 

机构地区：[1]中国农业大学生物学院,农业生物技术国家重点实验室,北京100193 [2]北京出入境检验检疫局食品安全检测中心,北京100026

出　　处：《生物物理学报》2010年第8期680-690,共11页Acta Biophysica Sinica

基　　金：国家高技术研究与发展项目(863项目)(2007AA021805)~~

摘　　要：趋磁细菌可以在细胞内合成Fe3O4或F3S4颗粒,称为磁小体。磁小体粒径为纳米级,晶形稳定,有质膜包被,在免疫检测、临床诊断、靶向治疗、污水处理等方面均有很好的应用前景。文章主要介绍采用化学法和生物法对磁小体进行修饰,并利用修饰后的磁小体对病原物进行免疫磁性分离、前期诊断和痕量检测的方法。Magnetotactic bacteria synthesize Fe3O4 or Fe3S4 particles in vivo,which are called magnetosome.Purified magnetosome are nano-sized and membrane-enclosed magnetic crystals with species-specific shape and size.They offer vast potential uses in immunoassay,diagnosis,targeting therapy,pollutant treatment and so on.Applications of magnetosome in magnetic immunoassay are briefly reviewed,including modification of magnetosome with chemical or biological methods,and their usage in immunomangtic separation and detection of pathogens.

关 键 词：磁小体 磁小体-抗体复合物 免疫磁性分离 免疫检测 

分 类 号：Q939.99[生物学—微生物学]