人脂肪来源间充质干细胞植入受损耳蜗后定向归巢并分化为毛细胞样细胞  

Human Adipose-derived Mesenchymal Stem Cells Engrafted into Deafened Mammalian Cochlea and Differentiated into Hair Cell-like Cells

作　　者：袁先道[1] 闫曦[2,3] 杨华[1] 高志强[1] 李康华[2,3] 韩钦[2,3] 卢姗[2,3] 陈晓巍[1] 亓放[1] 姜鸿[1] 赵春华[2,3] 

机构地区：[1]中国医学科学院北京协和医学院北京协和医院耳鼻咽喉科,北京100730 [2]中国医学科学院 [3]北京协和医学院基础医学院组织工程中心,北京100005

出　　处：《中国生物工程杂志》2010年第8期1-5,共5页China Biotechnology

基　　金：国家"十一五""863"计划重大专项(2006AA02A109;2006AA02A115);国家自然科学基金(30570771);北京市科委重点项目(D07050701350701);北京市科技新星计划(2006B53)资助项目

摘　　要：目的:通过将人脂肪来源间充质干细胞(human adipose-derived mesenchymal stem cells,hAD-MSCs)移植到受损小鼠,探讨hAD-MSCs替代缺失/受损毛细胞的可行性。方法:将hAD-MSCs经尾静脉移植入药物致聋后的小鼠体内,用免疫染色及RT-PCR等方法检测移植后hAD-MSCs在耳蜗内的归巢和分化。结果:移植的hAD-MSCs能够定向归巢到受损耳蜗内,并至少存活2周,未观察到对移植细胞的免疫排斥反应。有少量细胞定位于耳蜗感觉上皮并表达毛细胞特异性抗体myosin 7a。结论:hAD-MSCs移植入药物性致聋小鼠后,能够定向归巢到耳蜗内,并分化为内耳毛细胞样细胞,是一种针对内耳损伤及退变性疾病治疗的潜在细胞来源。Objective：The aim was to examine the potential of human adipose-derived mesenchymal stem cells （hAD-MSCs） transplantation to restore inner ear hair cells in mice. Methods： To test this approach,hAD-MSCs were delivered into the cochlea of deafened mice. The grafted cells were tested by immunostaining and RT-PCR. Results： Transplanted cells survived for at least 2 weeks postoperatively,evidenced by RT-PCR and immunofluorescence detection of hAD-MSCs. A few hAD-MSCs located in the cochlear sensory epithelium, with expression of myosin immunological rejectio ear after drug-induced 7a, a specific marker for inner ear hair n of the implanted hAD-MSCs. Conclusion： injury can survive, migrate and differentiate cells. There was no evidence of significant hAD-MSCs transplanted into the mouse inner towards hair cell-like cells.

关 键 词：耳蜗 间充质干细胞 耳聋 异种移植 

分 类 号：Q819[生物学—生物工程]