几丁质酶产生菌筛选鉴定及产酶性能研究  

Research on Screening ,Identification and Enzyme Producing Characteristics of Chitinase Producer

作　　者：张海涛[1] 王婷[1] 田囡[1] 刘新利[1] 

机构地区：[1]山东轻工业学院山东省微生物工程重点实验室,济南250353

出　　处：《中国生物工程杂志》2010年第8期82-87,共6页China Biotechnology

基　　金：山东省中青年科学家科研奖励基金(2007BSB01350);山东省教育厅科技计划(J07YGO4)资助项目

摘　　要：从土壤样品中筛选得到一株高产几丁质酶菌株C65-2,经形态学观察和18S rDNA序列测定,鉴定为Aspergillus fumigatus,对产酶培养基进行初步优化,测得最高酶活可达6.9U/ml,酶活力较优化之前提高了210%。酶学性质研究表明该几丁质酶分子量约为20kDa,酶在60℃下保温50min酶活降为0,最适酶反应温度是55℃,酶反应最适pH为7.0,Mg2+,Cu2+对酶反应有促进作用,Fe3+对酶反应有抑制作用。C65-2 strain with high chitinase producing ability was isolated from soil and identified it as Aspergillus fumigatus by observing its morphology character and measuring its 18S rDNA sequence. The chitinase producing conditions was optimized. The maximum activity could be up to 6.9 U/ml and the enzyme activity increased 210% than before. The study of enzymatic properties of the chitinase showed that the molecular weight was 20kDa. Thermostable stability of the chitinase was not good and the enzyme activity reduced to 0 at 60℃ for 50 min. The optimal enzyme reaction temperature was 55℃ and the optimal pH of enzyme reaction was 7.0. The chitinase activity can be enhanced by Mg2＋ ,Cu2＋ and inhibited by Fe3＋.

关 键 词：几丁质 几丁质酶 烟曲霉 产酶特征 

分 类 号：Q93-3[生物学—微生物学]