花生AhLEA18蛋白基因的克隆与表达分析  

Cloning and Expression Analysis of Peanut AhLEA18 Protein Gene

作　　者：苏磊[1,2] 姜娜娜[3] 王兴军[1,2] 赵传志[2] 毕玉平[1,2] 

机构地区：[1]山东师范大学生命科学学院,济南250014 [2]山东省农业科学院高新技术研究中心,农业部黄淮海作物遗传改良与生物技术重点开放实验室,山东省作物与畜禽品种改良生物技术重点实验室,济南250100 [3]山东现代职业学院,济南250100

出　　处：《中国农学通报》2010年第17期47-50,共4页Chinese Agricultural Science Bulletin

基　　金：山东省农业科学院创新基金项目"花生籽粒全长cDNA文库的大规模测序及脂肪酸合成关键酶基因分离和功能分析"(2006YCX030);"花生品质(脂肪酸;蛋白质)相关功能基因挖掘及高效转基因技术体系能研究"(2007YCX001);山东省自然科学基金项目"抑制PEPCase基因表达提高花生油份含量的研究"(Y2008D44)

摘　　要：LEA蛋白家族是一类在种子胚胎发育晚期丰富表达的蛋白,在种子的正常发育过程中大量积累,同时在植物处于干旱、寒冷、盐胁迫等逆境条件下高水平表达,推测该类蛋白在缺水条件下具有一定的生理保护功能。根据蛋白序列特点,该蛋白家族分为多个组,LEA18蛋白属于其中一组蛋白。PvLEA18蛋白在干燥种子、花粉粒中大量积累,也参与到对水分缺失、ABA处理的生理应答过程。该文从栽培种花生鲁花14中克隆得到一个LEA18基因,命名为AhLEA18。对其序列的生物信息学分析表明,AhLEA18具有典型LEA18蛋白序列特征。通过半定量RT-PCR的方法对该基因在花生植株不同组织及不同发育时期的种子中的表达情况进行了分析。In plants, a group of hydrophilic proteins, known as Late Embryogenesis Abundant （LEA） proteins, accumulate to high level during the late stage of seed development and during water deficiency in vegetative organs, suggesting a protective role during water limitation. LEA18 protein is a member of LEA protein family. ThePv LEA18 transcript and protein are highly accumulated in dry seeds and pollen grains and also respond to water deficit and ABA treatments. One LEA18 protein gene from peanut （Luhua 14） was cloned and named AhLEA18 . Aligning with several LEA18 proteins from other species by ClustalW program, the result demonstrated that theAh LEA18 is a typical LEA18 protein. The expression of this gene in different tissues and four developmental stages of peanut seeds were analyzed by Semi-quantitative RT-PCR.

关 键 词：花生 胚胎发育晚期丰富蛋白 花生LEA18 半定量RT-PCR 

分 类 号：S184[农业科学—农业基础科学]