氯化镧对原代大鼠星形胶质细胞凋亡和细胞周期的影响  

Effect of lanthanum chloride on apoptosis and cell cycle in primary cultured astrocytes of rat

作　　者：刘秋芳[1] 鲁帅[1] 齐鸣[1] 巫生文[1] 蔡原[1] 

机构地区：[1]中国医科大学公共卫生学院卫生毒理学教研室,辽宁沈阳110001

出　　处：《中国工业医学杂志》2010年第4期250-252,F0003,共4页Chinese Journal of Industrial Medicine

基　　金：国家自然科学基金资助项目(30800925)

摘　　要：目的研究不同浓度的氯化镧对原代大鼠星形胶质细胞凋亡和细胞周期的影响,探讨氯化镧所致神经损伤的机制。方法取新生大鼠大脑皮质进行原代星形胶质细胞分离和培养。在纯化及鉴定细胞后,用0,0.25,0.5,1.0 mmol/L氯化镧(LaC l3)处理24 h后,应用MTT法检测细胞活力,比色法检测乳酸脱氢酶(LDH)释放,应用倒置相差显微镜观察细胞形态,流式细胞仪法检测细胞凋亡和细胞周期。结果随着LaC l3暴露浓度的增加,细胞活力明显降低,LDH释放明显增加,细胞形态出现不同程度的损伤,细胞凋亡率从0.72%上升至11.6%(P<0.05),G1期细胞指数从78.35%上升至92.11%(P<0.05),S期细胞指数从6.19%下降至1.53%(P<0.05),G2期细胞指数从15%下降至7.09%(P<0.05),均呈现剂量-效应关系。结论氯化镧可诱发原代大鼠星形胶质细胞凋亡,改变细胞周期,从而产生神经毒性。Objective To study the effect of lanthanum chloride（LaCl3） on apoptosis and cell cycle in the primary cultured astrocytes of rat for exploring its mechanism of neurotoxicity.Methods The primary cultured astrocytes from neonate rat were separated and cultured,then exposed to 0,0.25,0.5 or 1.0 mmol/L of LaCl3 for 24h after purification and identification.The cell viability was determined by MTT assay,and the apoptosis and cell cycle were determined by flow cytometry.Results The cell viability in the astrocytes exposed to LaCl3 decreased significantly from 100%（as controls） to 53.31%（P0.01） with the increase of exposure dose of LaCl3,the rate of apoptosis increased significantly from 0.72% to 11.6%（P0.05）,meanwhile,there were some abnormalities in cellular appearance.On the other hand,the percentage of cells in G1 phase was elevated significantly from 78.35% to 92.11%（P0.05）,that in S phase reduced significantly from 6.19% to 1.53%（P0.05）,and that in G2 phase decreased significantly from 15% to 7.09%（P0.05）,which all were dose dependent.Conclusions The results suggested that LaCl3 exposure may induce apoptosis and abnormal cell cycle in cultured astrocytes,which might be the possible mechanism of neurotoxicity by LaCl3.

关 键 词：氯化镧 细胞凋亡 细胞周期 星形胶质细胞 

分 类 号：O612.3[理学—无机化学] R995[理学—化学]