HPLC法测定导赤散及配伍组血浆中马兜铃酸A含量  

Determination of Aristolochic Acid A in Plasma of Daochi Powder and Compatible Drug Groups by HPLC

作　　者：吴建红[1] 李汉鑫[1] 吴嫦[1] 

机构地区：[1]湖北中医药大学,湖北武汉430065

出　　处：《湖北中医学院学报》2010年第4期30-32,共3页Journal of Hubei College of Traditional Chinese Medicine

基　　金：湖北中医药大学校课题(中医科(2005)11号)

摘　　要：目的通过检测导赤散及配伍组血浆中马兜铃酸A的含量,探索导赤散配伍减毒的作用和马兜铃酸A在大鼠体内代谢的时量关系。方法采用HPLC法,色谱柱为Diamon TMC18(250mm×4.6mm,5μm),甲醇-水-冰乙酸(72∶27∶1)为流动相;室温;检测波长为315nm;流速为1.0mL/min。大鼠随机分5组(导赤散组、配伍一组、配伍二组、关木通组和空白对照组),按0.054g/kg的中药煎液灌胃给药。第7天、第15天后采血制备血浆。经HPLC法测定上述5组血浆中马兜铃酸A的含量。结果给药7d后,导赤散组、配伍一组和配伍二组血浆中均未检测出马兜铃酸A,关木通组血浆中马兜铃酸A的含量为63.386μg/mL;给药15天后,关木通组、配伍一组和配伍二组血浆中马兜铃酸A的含量分别为:99.890、28.824、50.156μg/mL。导赤散组血浆中马兜铃酸A的含量低于最低检测限。结论导赤散的配伍减毒作用优于配伍一组、配伍二组;马兜铃酸A在大鼠体内的代谢存在时量关系。Objective To compare with the contents of aristolochic acid A in plasma of Daochi powder and compatible drug groups,and to explore the poison reducing effects and time-concentration relationship of them.Methods HPLC was applied in this study.The analytical column was Diamon TMC18 column（250mm×4.6mm,5μm）,the mobile phase consisted of a mixture,methanol-water-acetic acid（72 ∶ 27 ∶ 1） at room temperature.The detecting wavelength was 315nm.The flow rate was 1.0ml/min.Rats were divided randomly into five groups： Daochi powder group,compatible drug groups I and II,Caulis Aristolochiae Manshuriensis group and blank control group.After 7 and 15 days,the blood of all rats in the above five groups were collected and prepared,which afterwards underwent HPLC.Results After 7 days,the content of aristolochic acid A in plasma of Caulis Aristolochiae Manshuriensis group was 63.386 μg/mL,the rest groups were detected non-obvious aristolochic acid A.After 15 days,the contents of aristolochic acid A in plasma of Caulis aristolochiae Manshuriensis group,compatible drug group I and II were 9.890,28.824,50.156 μg/mL respectively.The aristolochic acid A content was detected lower than the limit of detection.Conclusion The toxic attenuated effect of Daochi powder enables the advantage of compatible drug group I and II obviously.There was a time-concentration relationship in the rats metabolism of aristolochic acid A in vivo.

关 键 词：马兜铃酸A 血药浓度 关木通 导赤散 配伍 HPLC 

分 类 号：R285.1[医药卫生—中药学]