HPLC法测定肝舒胶囊中大黄素的含量  

HPLC for Determination of Emodin Content in Ganshu Capsule

作　　者：陈运初 

机构地区：[1]湖北省红安县七里坪镇中心卫生院,湖北红安438400

出　　处：《湖北中医学院学报》2010年第4期40-42,共3页Journal of Hubei College of Traditional Chinese Medicine

摘　　要：目的建立HPLC法测定肝舒胶囊中大黄素含量的方法。方法采用色谱柱Sino Chrom ODS(4.60 mm×250 mm,5μm);流动相为甲醇-0.1%磷酸溶液(85∶15);检测波长λmax=254nm。结果大黄素线性范围为4.12-46.16μg/mL,r=0.9999;平均加样回收率为99.75%,RSD为1.03%。结论本法简便、准确、灵敏度高、重现性好,可用于该制剂的含量测定。Objective To establish a method of HPLC determination of emodin in Ganshu capsules.Method HPLC analysis is carried out on Sino Chrom ODS（250×4.60mm,5μm） column;mobile phase： methanol-0.1%phosphoric acid solution（85 ∶ 15）.The detection wavelength was 254nm.Result The method was linear within the range of 4.12-46.16μg/mL,r=0.9999,and the average recovery rate of emodin was 99.75%,RSD was 1.03%.Conclusion The method is simple,accurate,highly sensitive and reproducible,therefore may be used for the quantitative determination of emodin in Ganshu capsules.

关 键 词：肝舒胶囊 大黄素 高效液相色谱法 含量测定 

分 类 号：R283.65[医药卫生—中药学]