在哺乳细胞中构建不同MicroRNA载体的研究  

The Research on Construction the Different MicroRNA Vectors in Mammals

作　　者：曾洁琼[1] 王峰尖[1] 潘坤[1] 向波[1] 

机构地区：[1]郧阳师范高等专科学校化学系,丹江口442700

出　　处：《生物技术通报》2010年第9期25-27,共3页Biotechnology Bulletin

基　　金：郧阳师专2009年教学改革研究项目(2009117)

摘　　要：RNA干扰技术是分子生物学研究的热点,同时也是有效阐明基因功能的有力工具。利用载体表达的小干扰RNA在疾病的治疗研究中意义更为重要。针对RNA干扰技术的发展,构建载体的研究及其应用前景作了简单概述,对如何在哺乳动物中构建不同microRNA载体作了详细报道。RNA interference technique is identified as a focus in molecular biology,which is also envisioned to be a useful tool to elucidate gene function.Using vectors to produce shRNA is desired to be a more important approach in disease therapeusis.In this paper,a presentation of the research on the development of RNA interference technique,the vectors construction and the prospective of applications were briefly summarized.A method for constructing the different microRNA vectors in mammals was reviewed.

关 键 词：分子生物学 RNA干扰技术 基因 疾病的治疗 

分 类 号：Q78[生物学—分子生物学]