肠道特异性基因表达调控元件研究进展  

Research on Intestinal Gene Specific Expression Regulatory Elements

作　　者：翟亚峰[1] 束刚[1] 张志岐[1] 王松波[1] 江青艳[1] 

机构地区：[1]华南农业大学动物科学学院,广州510642

出　　处：《生物技术通报》2010年第9期32-37,共6页Biotechnology Bulletin

基　　金：国家转基因生物新品种培育科技重大专项(2008ZX08006-004);国家自然科学基金委-广东联合基金重点项目(u0731004)

摘　　要：外源性基因在特定靶组织的高效、稳定表达是转基因动物研究和基因治疗的重要前提。外源基因在非靶组织中的异位表达一直是制约基因治疗技术的发展,影响转基因动物的安全性的重要因素。目前,一系列肠道特异性基因表达调控序列相继被克隆并鉴定。就上述调控序列的结构、功能和转录活性以及组织特异性作简要综述,为构建稳定、高效、特异性的嵌合型肠道转录调控元件提供理论依据,对环境友好型转基因动物的生产以及肠道性疾病有效的基因治疗研究具有重要参考价值。The stable and efficient expression of the exogenous gene in specific target tissues is an important prerequisite for gene therapy and transgenic animals research.The development of gene therapy technology and transgenic animals safety were highly restricted when exogenous genes ectopic expression happened in the non-target tissue.A series of intestinal specific regulatory elements have been cloned and characterized.In this paper,the construction,function,and tissue specificity of intestinal regulatory elements are reviewed to provide theories for constructing the intestinal regulatory elements chimeras.This provides fundamental references for producing environmental-friendly transgenic animals and efficacy of intestinal diseases gene therapy.

关 键 词：肠道 表达调控元件 特异性 基因治疗 转基因动物 

分 类 号：Q75[生物学—分子生物学]