箭筈豌豆两个肌动蛋白基因片段的克隆及序列分析  

Cloning and Sequencing of Two Actin Genes from Vicia sativa

作　　者：张磊[1] 刘志鹏[1] 张吉宇[1] 张妙青[1] 王彦荣[1] 

机构地区：[1]兰州大学草地农业科技学院,兰州730000

出　　处：《生物技术通报》2010年第9期70-75,共6页Biotechnology Bulletin

基　　金：国家基础研究发展计划"973"项目(2007CB108904);兰州大学中央高校基本科研业务费专项资金项目(lzujbky-2009-4);"十一五"国家科技支撑计划子课题(2008BADB3B03-4)

摘　　要：通过同源克隆获得了箭筈豌豆两个不同的Actin基因片段,为研究该箭筈豌豆其它基因的表达状况提供了内标参照。根据近缘物种Actin基因序列设计一对引物,通过RT-PCR技术分别从叶片和果实材料中克隆获得了两条不同的Actin基因片段。生物信息学分析表明,叶片中克隆的片段长度604 bp,编码201个氨基酸;果实中克隆的片段长度677 bp,编码225个氨基酸。两条序列与其它物种Actin基因的碱基序列相似度高于80%,氨基酸序列相似度高于93%。将来自叶片和果实的两条序列分别命名为VsACT7和VsACT11,并在GenBank注册,登录号分别为HM004434和GU946218。To provide internal standard for expression analysis of other genes,a partial sequence of actin gene was isolated from common vetch（Vicia sativa）.In this study,a pair of primers was designed according to homologous actin genes among closely related plants,and then the reverse transcription PCR（RT-PCR） was performed.Then two different sequences were deprived from leaf and fruit,respectively.Homological analysis showed that the similarity between two sequences and other actin sequences were over 80% in nucleoride level,while 93% in animo acid level.So the two sequences were considered to be different copies of actin gene in Vicia sativa.They were named as VsACT7 and VsACT11 respectively,and submited to GenBank（accession number：HM004434,GU946218）.

关 键 词：箭筈豌豆 ACTIN基因 RT-PCR 同源克隆 

分 类 号：Q943.2[生物学—植物学] S542[农业科学—作物学]