透明颤菌血红蛋白在枯草芽孢杆菌WB800中的表达及其对菌体生长的影响  

Expression of Vitreoscilla Hemoglobin Gene in Bacillus subtilis WB800 and Effects on Growth

作　　者：廖瑜玲 任楠[2] 李俊星[2] 杨慧林[2] 

机构地区：[1]广东肇庆星湖生物科技股份有限公司,肇庆526040 [2]华南理工大学生物科学与工程学院,广州510006

出　　处：《生物技术通报》2010年第9期177-180,共4页Biotechnology Bulletin

摘　　要：通过基因工程的方法,将透明颤菌血红蛋白基因(vhb)置于大肠杆菌-枯草杆菌穿梭质粒pBEP43-DFE的强启动子P43下,构建重组质粒pBEP43-vhb,并通过化学感受态法转入枯草杆菌WB800中,转化子经过酶切和PCR验证。采用一氧化碳差异色谱法测定转入的血红蛋白基因具有表达活性。通过限氧试验表明,血红蛋白基因能在低氧环境下促进菌体的生长。Through genetic engineering method,the Vitreoscilla hemoglobin gene（vhb） was inserted into the Escherichia coli-Bacillus subtilis shuttle vector pBEP43-DFE.The recombinant plasmid pBEP43-vhb was constructed and chemically transformed into Bacillus subtilis WB800.Molecular analysis of the recombinant was carried by enzyme digestion and PCR.The Vitreoscilla hemoglobin gene was demonstrated by carbon monoxide binding analysis,and the result show that active Vitreoscilla hemoglobin was expressed in Bacillus subtilis WB800.According to limited oxygen experiment,Vitreoscilla hemoglobin enhanced cell growth under the low dissolved oxygen concentrations.

关 键 词：透明颤菌 血红蛋白 枯草芽孢杆菌 表达 生长 

分 类 号：Q78[生物学—分子生物学]