液质联用法快速测定人血浆福辛普利拉浓度  

Rapid Determination of Fosinoprilat in Human Plasma by LC-MS/MS

作　　者：罗金文[1] 郑国钢[1] 李会林[1] 

机构地区：[1]浙江省食品药品检验所,杭州310004

出　　处：《医药导报》2010年第9期1129-1132,共4页Herald of Medicine

摘　　要：目的建立快速测定人血浆福辛普利的活性代谢物福辛普利拉浓度的液质联用法。方法采用LunaC18(2.1mm×150mm,3.0μm);流动相为甲醇-乙腈-0.025%氨水(30:30:40),流速0.2mL.min-1,质谱条件为电喷雾电离源(ESI-),以选择反应离子监测(SRM)方式进行检测,用于定量分析的反应离子为m/z434.3→237.0。结果福辛普利拉在0.25～400.00ng·mL-1范围内线性关系良好。福辛普利拉的日内、日间RSD均<10%。福辛普利拉的最低检测浓度为0.05ng·mL-1。结论该方法分析速度快,专属性强,灵敏度高,测定结果准确,可用于福辛普利的人体药动学研究。Objective To establish a LC-MS/MS method for the determination of fosinoprilat,an active metabolite of fosinopril in the human plasma. Methods The separation and analysis was performed on a Luna C18 column（2.1 mm×150 mm,3.0 μm）.The mobile phase consisted of methanol-acetonitrile-0.025% ammonia water （30：30：40）.The flow rate was 0.2 mL·min-1. Electrospray ionization（ESI） source was applied and operated in the negative mode. Selected reaction monitoring（SRM） mode with the transitions of m/z 434.3→237.0 was used to quantify fosinoprilat. Results The assay linearities of fosinoprilat was confirmed over the range 0.25～400.00 ng·mL-1,and the detection limit was 0.05 ng·mL-1. The intra and inter-day precision （RSD） were less than 10%. Conclusion The method is proved to be rapid,specific,sensitive and accurate,which can be used for the clinical pharmacokinetic studies of fosinopril.

关 键 词：福辛普利 福辛普利拉 液质联用 药动学 

分 类 号：R972.4[医药卫生—药品] R969.1[医药卫生—药学]