聚合酶链反应检测HBV—DNA与HBV血清学标志的关系  

Relationship between Serological Markers of HBV-DNA and HBV Detected by Polymerase Chain Reaction

作　　者：吴淑华[1] 钱霞[1] 

机构地区：[1]江苏省泰兴市卫生防疫站

出　　处：《实用预防医学》1999年第2期81-83,共3页Practical Preventive Medicine

摘　　要：目的探讨聚合酶链反应检测ＨＢＶ—ＤＮＡ与ＨＢＶ不同血清学标志中的关系。方法采用聚合酶链反应（ＰＣＲ）技术对２７０例ＨＢＶ不同血清学标志及不同组合进行了ＨＢＶ—ＤＮＡ的检测。结果ＨＢＶ—ＤＮＡ在ＨＢｅＡｇ标志中检出率最高，阳性率为１００％；其次是ＨＢｓＡｇ、抗ＨＢｃ和抗—ＨＢｅ，阳性率分别为６６０％、６５９％和４６２％；在抗—ＨＢｓ标志物中未能检出ＨＢＶ—ＤＮＡ；在ＨＢｓＡｇ滴度为≤５１２、＞５１２和阴性者中阳性率分别为６０６％、７５７％和２１９％；在ＡＬＴ异常和正常者中阳性率分别为６８８％和２９５％。结论ＨＢＶ—ＤＮＡ在ＨＢＶ血清学标志不同组合、不同ＨＢｓＡｇ滴度和不同肝功能中的阳性率有着非常显著性差别。Objective To explore the relationship between different serological markers of HBV-DNA and HBV detected by Polymerase Chain Reaction(PCR).Methods The PCR was used to detect the different serological markers and different combination of 270 cases of HBV. Results The highest detective rate of HBsAg markers was HBV-DNA,which was 100%,and followed by the HBsAg,anti-HBC and anti-HBe with the detective rate of 66.0%,65.9% and 46.2% respectively.The HBV-DNA was not detected.Among the cases with the titre of HBsAg≤512,>512 and negative,the positive rates were 6.06%,75.7% and 21.9% respectively. The positive rates of cases with abnormal and normal ALT were 68.8% and 29.5% respectively. Conclusion There was significant difference of the positive rate of HBV-DNA among the cases with different combination of HBV serological marker, cases with different HBsAg titre and cases with different hepatic function. [

关 键 词：聚合酶链反应 HBV-DNA 血清学标志 

分 类 号：R446.112[医药卫生—诊断学]