聚乙二醇模拟干旱对甜高粱幼苗丙二醛、脯氨酸含量的影响  

Effects of Drought Stress Simulated by PEG on Malonaldehyde and Proline Contents of Sweet Sorghum Seedlings

作　　者：董喜存[1] 李岩[2] 李文建[1] 马晓琪[1] 冯亮英[2] 何金玉[1] 曲颖[1] 刘青芳[1] 严翔[3] 

机构地区：[1]中国科学院近代物理研究所,甘肃兰州730000 [2]兰州大学生命科学学院,甘肃兰州730000 [3]兰州大学第一附属医院,甘肃兰州730000

出　　处：《云南农业大学学报（自然科学版）》2010年第5期726-730,736,共6页Journal of Yunnan Agricultural University:Natural Science

基　　金：甘肃省中青年基金项目(099RJYA012);中国科学院科技支甘项目(0806300YDO);中国科学院西部之光项目(0906040XBO);中国科学院方向性重点项目(KJCX2-YW-N34-3)

摘　　要：以甜高粱品种KFJT-CK及经过碳离子辐照选育出的早熟突变株KFJT-1为材料,用浓度分别为5%,10%和15%的聚乙二醇(PEG)6000模拟干旱对其进行胁迫处理,测定丙二醛(MDA)及脯氨酸(Pro)的含量。随着胁迫时间的延长和胁迫程度的增加,MDA含量持续升高;Pro含量在5%和10%PEG胁迫下持续升高,在15%PEG胁迫下先升高后降低。表明碳离子辐照可能使甜高粱膜脂过氧化特性发生改变,影响Pro的表达。为进一步研究碳离子束辐照对甜高粱的耐旱生理提供一定的基础,并为下一步的育种工作提供有用的参考。The contents of malonaldehyde（MDA） and proline（Pro） were measured in seedlings of sweet sorghum KFJT-CK and its premature mutant progeny KFJT-1 treated with 5%,10% and 15% polyethylene glycol（PEG） 6000.After drought stress,MDA content in sweet sorghum seedling rapidly and persistently increased;the higher the PEG concentration was,the higher the MDA content was.Under 5% and 10% PEG stress,Pro content got more and more along with days and PEG concentration increase.However,under 15% PEG,Pro content firstly increased and then decreased.MDA contents had no apparent difference between KFJT-CK and KFJT-1 seedlings,but Pro content had remarkable difference between KFJT-CK and KFJT-1 seedlings.Carbon ion irradiation affected the peroxidation characteristics of membrane lipid and the Pro expression of sweet sorghum.This paper provides a foundation for further study on drought-stressed physiology by carbon ion irradiation in sweet sorghum and some reference for the following breeding work.

关 键 词：聚乙二醇 甜高粱 丙二醛 脯氨酸 

分 类 号：Q514.035.2[生物学—生物化学]