九台晚李PGIP基因的克隆及生物信息学分析  

Cloning and Bioinformatic Analysis of PGIP Gene from Jiutaiwanli

作　　者：郭庆勋[1] 张春雨[1] 王晶莹[1] 周连霞[1] 王彦涛[1] 

机构地区：[1]吉林大学植物科学学院,长春130062

出　　处：《植物遗传资源学报》2010年第5期650-653,共4页Journal of Plant Genetic Resources

基　　金：吉林大学农学部博士启动基金项目(430505010203);长春市科技局国际合作计划项目(2D5070046202)

摘　　要：以九台晚李叶片基因组为模板,PGIP基因保守序列设计引物,PCR扩增到1条全长1192bp的目的片段（GenBank登录号：GU068978）。该基因包含有1个完整的开放阅读框,由2个外显子和1个内含子构成,外显子总长990bp,编码330个氨基酸,其编码的氨基酸序列中含有一段典型的亮氨酸重复序列。序列分析表明：该基因与中国李、杏、桃、马哈利樱桃、梅等李属植物的PGIP基因序列一致度达95%-99%。系统进化分析显示出属内亲缘关系较近、属间亲缘关系较远的特点。该序列为植物分子抗病育种提供了1条新的基因资源。A target fragment with a full length of 1192 bp（GenBank accession：GU068978） was amplified with genomic DNA of Prunus salicina L.cv.Jiutaiwanli leaves as the templates and the conservative sequences of PGIP gene as the primers.This sequence had a full open reading frame encoding the polygalacturonase-inhibiting protein,and eontained two exons interrupted by one intron.The total exons were comprised by 990bp of deoxynucleotide encoding 330 amino acid.A conserved leucine-rich fragmenthad existed in the derived protein sequence.Sequencing analysis showed that it was 95% to 99% identical with the sequences of Prunus PGIP genes including P.salicina,P.armeniaca,P.persica,P.mahaleb,P.mume.Phylogenic tree showed that genetic relationship within the genus was closer and between the genera was farther.As a result,a gene resource was provided for molecular breeding of plants.

关 键 词：九台晚李 PGIP基因 基因克隆 生物信息学 

分 类 号：S662.3[农业科学—果树学]