双腧穴针刺对SD大鼠热水甩尾痛阈的影响  

Study about Pain-Threshold of Tail-flick Latency by Acupuncturing Double Points on SD Rats

作　　者：谈建新[1] 孟凡迅[2,3] 季进锋[3] 林社裕[1] 黄凯[1] 常爽[1] 吕雪娇[1] 崔阳艳[1] 

机构地区：[1]江苏南通大学生命科学学院,江苏南通226007 [2]江苏南通大学神经科学系,江苏南通226001 [3]南通大学附属肿瘤医院,江苏南通226361

出　　处：《辽宁中医药大学学报》2010年第9期65-68,共4页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：江苏省教育厅自然科学研究基金资助项目(04KJB360115);南通大学自然科学研究基金资助项目(09ZY006)

摘　　要：目的:通过针刺SD大鼠单个腧穴和双腧穴热水甩尾痛阈值的观察和分析,探讨针刺腧穴的组方,对大鼠针刺镇痛模型的作用,为深入研究临床针刺用穴处方机理提供基础实验条件。方法:分别单针针刺SD大鼠"后三里"、"关元"和双针同时针刺"后三里"+"后三里"、"后三里"+"关元"等腧穴,采用热水甩尾测痛方法,测量大鼠针刺前后的痛阈,并对实验结果进行统计学处理。结果:(1)单针针刺"后三里"腧穴,针刺前(5.6301±0.3296)针刺后(10.0680±0.4403)配对t检验比较差异显著(P=0.0001);单针针刺"关元"腧穴,针刺前(5.3511±0.1860)针刺后(7.5133±0.2615)配对t检验比较差异显著(P=0.0001);双针针刺"后三里"+"后三里"腧穴,针刺前(5.2167±0.3513)针刺后(10.5850±0.6087)配对t检验比较差异显著(P=0.0001);双针针刺"后三里"+"关元"腧穴,针刺前(4.8000±0.3061)针刺后(9.4979±0.6233)配对t检验比较差异显著(P=0.0001);(2)单针针刺"后三里"针刺后(9.8293±0.3469)和双针针刺"后三里"+"后三里"腧穴针刺后(10.1936±0.5839)配对t检验比较未见有统计学意义的改变(P=0.5455);单针针刺"关元"针刺后(7.5133±0.2615)与双针针刺"后三里"+"关元"腧穴针刺后(9.4979±0.6233)配对t检验比较未见有统计学意义的改变(P=0.7579);双针针刺"后三里"+"后三里"腧穴针刺后(10.1936±0.5839)与双针针刺"后三里"+"关元"腧穴针刺后(9.4979±0.6233)配对t检验比较未见有统计学意义的改变(P=0.2436);单针针刺"关元"针刺后(7.5133±0.2615)和双针针刺"后三里"+"关元"腧穴针刺后(9.4979±0.6233)配对t检验比较,两者之间显示出有统计学意义的差异(P=0.0420);(3)双针针刺"后三里"+"后三里"腧穴针刺前痛阈值(5.2167±0.3513)与针刺后60min痛阈值(6.1308±0.5036)比较,未见有统计学意义的改变(P=0.1279);单针针刺"关元"腧穴针刺前(5.6429±0.3253)与针刺后60min痛阈值(5.6601±0.5129)比较,未见有统计学意义的改变(P=0.9703);单�Objective：To investigate the effect of acupuncture analgesia models with different acupuncture point prescriptions and provide the scientific experimental foundation for further study on the mechanism of acupuncture point prescriptions with the method of analysing the tail-flick latency by Acupuncturing Double Points on the SD Rats.Method：We choose single point HOUSANLI or GUANYUAN,double points HOUSANLI with HOUSANLI or HOUSANLI with GUANYUAN.Various acupuncture point prescriptions are used to gather Different datas of pain-threshold with the methods of investigating tail-flick latency,and the datas are analyzed statistically.Result：（1）With T-test,the pain-threshold on HOUSANLI Pre-and-Post acupuncture is significantly different（P=0.0001）,the pain-threshold on GUANYUAN is also significantly different（P=0.0001）.What＇s more,the pain-threshold on HOUSANLI with HOUSANLI points and HOUSANLI with GUANYUAN points Pre-and-Post double acupuncture are both significantly different（P=0.0001）.（2）With T-test,the pain-threshold between single HOUSANLI point acupuncture（9.8293±0.3469）and HOUSANLI with HOUSANLI points double acupuncture（10.1936±0.5839）isn＇t statistically significant（P=0.5455）.The pain-threshold between acupuncture single GUANYUAN point（7.5133±0.2615）and acupuncture HOUSANLI with GUANYUAN points（9.4979±0.0.6233）is also not statistically different（P=0.7579）.The pain-threshold between HOUSANLI with HOUSANLI points after double acupuncture（10.1936±0.5839）and HOUSANLI with GUANYUAN points after double acupuncture（9.4979±0.6233）,there is as well no statistical significance（P=0.2436）.The pain-threshold on GUANYUAN point after single acupuncture（7.5133±0.2615）is significantly different（P=0.0420）from that HOUSANLI with GUANYUAN points after double acupuncture（9.4979±0.6233）.（3）The pain-threshold of double acupuncture HOUSANLI with HOUSANLI points before（5.2167±0.3513）compared with acupuncture after sixty minutes（6.1308±0.503

关 键 词：腧穴 针刺镇痛 甩尾测痛 痛阈模型 

分 类 号：R245.31[医药卫生—针灸推拿学]