硫鱼精蛋白去除OPV中Vero细胞基质DNA的研究  

Study on Removing Residual DNA of Vero Cells in OPV with Protamine Sulphate

作　　者：廖国阳[1] 姜述德[1] 陈巍[1] 王燕[1] 史荔[1] 马波[1] 余芬[1] 

机构地区：[1]中国医学科学院中国协和医科大学医学生物学研究所

出　　处：《云南大学学报（自然科学版）》1999年第3期192-194,共3页Journal of Yunnan University(Natural Sciences Edition)

摘　　要：以不同浓度的硫鱼精蛋白（ＰＳ）对Ｖｅｒｏ细胞制备的Ⅰ，Ⅱ，Ⅲ型脊髓灰质炎口服活疫苗（ＯＰＶ）病毒悬液进行后处理去除细胞基质ＤＮＡ研究．ＰＳ浓度大于１ｍｇ／ｍＬ，纯化后疫苗中ＤＮＡ残余含量低于１００ｐｇ／剂量．随着病毒液中ＰＳ浓度的增加，ＤＮＡ残余含量减少，但病毒回收率亦降低．结果表明ＰＳ沉淀法，病毒回收率高，ＤＮＡ残余含量能达到ＷＨＯ规程ＤＮＡ限量要求，对脊灰病毒ｒｃｔ／４０特征及病毒壳蛋白等生物学性状无显著影响。The effect of different concentrations of PS on the removal of cellular DNA from types Ⅰ, Ⅱ and Ⅲ OPV produced on Vero cells was examined.It was found that 1?mg/mL PS was able to remove residual cell DNA to meet the WHO requirements (content of DNA <100?pg/dose) and when PS concentrati on was increased,the DNA content and virus recovery rate decreased.It also show ed that the treatment of OPV with PS has no influence on rct/40 character and vi ral capsid.We demonstrated that precipitation of OPV with PS is simple method f or eliminating residual cellular DNA in OPV preparation on Vero cell.

关 键 词：脊髓灰质炎 硫鱼精蛋白 OPV 疫苗 VERO细胞 

分 类 号：R392－33[医药卫生—免疫学] R512.401[医药卫生—基础医学]